Detailed information of TA system TA238587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 6256732..6257324
• Replicon: chromosome
• Accession: NZ_CP093428
• Organism: Pseudomonas migulae strain 8R6

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: MOQ58_RS28640
• Protein ID: WP_084320214.1
• Coordinates: 6257046..6257324 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: J2PV29
• Locus tag: MOQ58_RS28635
• Protein ID: WP_007980826.1
• Coordinates: 6256732..6257037 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOQ58_RS28605 (MOQ58_28565)	6252295..6252618	-	324	WP_192416627.1	transcriptional regulator SutA	-
MOQ58_RS28610 (MOQ58_28570)	6252720..6253145	-	426	WP_008030499.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
MOQ58_RS28615 (MOQ58_28575)	6253359..6254696	-	1338	WP_008030500.1	ammonium transporter	-
MOQ58_RS28620 (MOQ58_28580)	6254733..6255071	-	339	WP_002555808.1	P-II family nitrogen regulator	-
MOQ58_RS28625 (MOQ58_28585)	6255490..6255750	+	261	WP_008030504.1	accessory factor UbiK family protein	-
MOQ58_RS28630 (MOQ58_28590)	6256153..6256680	+	528	WP_280162487.1	Bro-N domain-containing protein	-
MOQ58_RS28635 (MOQ58_28595)	6256732..6257037	-	306	WP_007980826.1	HigA family addiction module antitoxin	Antitoxin
MOQ58_RS28640 (MOQ58_28600)	6257046..6257324	-	279	WP_084320214.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MOQ58_RS28645 (MOQ58_28605)	6257920..6259413	+	1494	WP_223415410.1	YifB family Mg chelatase-like AAA ATPase	-
MOQ58_RS28650 (MOQ58_28610)	6259419..6260630	-	1212	WP_280162488.1	aldose 1-epimerase family protein	-
MOQ58_RS28655 (MOQ58_28615)	6260897..6262273	+	1377	WP_280162489.1	SrfA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10539.15 Da
• Isoelectric Point: 7.6732

>T238587 WP_084320214.1 NZ_CP093428:c6257324-6257046 [Pseudomonas migulae]
MIVSFKCSETEYLFCNGKTRLWSAILSVAERKLTMLDDAVVLKDLRSPPGNRLEALEGNRKGQHSIRINAQWRICFVWGC
NGPENVEIVDYH

Nucleotide

• Length: 279 bp

>T238587 NZ_LR890405:4438407-4438664 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11245.97 Da
• Isoelectric Point: 6.4556

>AT238587 WP_007980826.1 NZ_CP093428:c6257037-6256732 [Pseudomonas migulae]
MTKNGMRPVHPGEILKEEYLEPLSLTAAALARALNVSTPTVNDIVLQRRGVSADMALRLSICLDTTPEFWLNLQSTYDLR
KAEIERGAAIRDQVERLAHCA

Nucleotide

• Length: 306 bp

>AT238587 NZ_LR890405:4438676-4439221 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Antitoxin

• AlphaFold DB: J2PV29