Detailed information of TA system TA238473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2864540..2865267
• Replicon: chromosome
• Accession: NZ_CP093402
• Organism: Salmonella enterica subsp. enterica serovar Agona strain R21.0464

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V7IQW2
• Locus tag: MOP58_RS14150
• Protein ID: WP_000558158.1
• Coordinates: 2864540..2864851 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MOP58_RS14155
• Protein ID: WP_000561389.1
• Coordinates: 2864848..2865267 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOP58_RS14115 (2859707)	2859707..2860429	-	723	WP_000182066.1	SPI-1 type III secretion system guanine nucleotide exchange factor SopE2	-
MOP58_RS14120 (2861114)	2861114..2861509	+	396	WP_000422887.1	DUF1398 domain-containing protein	-
MOP58_RS14125 (2861838)	2861838..2862314	+	477	WP_000030944.1	hypothetical protein	-
MOP58_RS14130 (2862424)	2862424..2862618	+	195	WP_023134800.1	hypothetical protein	-
MOP58_RS14135 (2862981)	2862981..2863763	+	783	WP_001747695.1	DUF2971 domain-containing protein	-
MOP58_RS14140 (2863817)	2863817..2864032	-	216	WP_001747696.1	hypothetical protein	-
MOP58_RS14145 (2864062)	2864062..2864361	-	300	WP_000775225.1	hypothetical protein	-
MOP58_RS14150 (2864540)	2864540..2864851	+	312	WP_000558158.1	type II toxin-antitoxin system HigB family toxin	Toxin
MOP58_RS14155 (2864848)	2864848..2865267	+	420	WP_000561389.1	helix-turn-helix domain-containing protein	Antitoxin
MOP58_RS14160 (2865523)	2865523..2866017	+	495	WP_016419152.1	glycosyltransferase	-
MOP58_RS24410 (2866014)	2866014..2866172	+	159	WP_023165344.1	lipopolysaccharide 1,2-N-acetylglucosaminetransferase	-
MOP58_RS14165 (2866408)	2866408..2866827	-	420	WP_000354404.1	GNAT family N-acetyltransferase	-
MOP58_RS14170 (2867198)	2867198..2867467	+	270	WP_077905753.1	hypothetical protein	-
MOP58_RS14175 (2867633)	2867633..2867773	+	141	WP_000489598.1	hypothetical protein	-
MOP58_RS14180 (2867919)	2867919..2868461	-	543	Protein_2787	IS256 family transposase	-
MOP58_RS14185 (2868481)	2868481..2868573	-	93	WP_231923105.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2867919..2868596	677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.19 Da
• Isoelectric Point: 10.0279

>T238473 WP_000558158.1 NZ_CP093402:2864540-2864851 [Salmonella enterica subsp. enterica serovar Agona]
MHVISKEPFDEAARRYPNDSLAIKALYRLVREKDFSSPAELRTVIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFINKRF
YVKHIATHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T238473 NZ_LR890360:c2550581-2549259 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGAGGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGGTTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCTATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15301.49 Da
• Isoelectric Point: 4.6286

>AT238473 WP_000561389.1 NZ_CP093402:2864848-2865267 [Salmonella enterica subsp. enterica serovar Agona]
MIANTAKAIEATKALVAAVPFLGGSASEKDYRDALALVDYLIENDDENPLIDFLASKIAEYEDNSKQFAEFNKSVAEMPV
GVALLRTLIDQYKLSYSDLKEEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT238473 NZ_LR890360:c2550847-2550581 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACATGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: V7IQW2