Detailed information of TA system TA238441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5676650..5677245
• Replicon: chromosome
• Accession: NZ_CP093395
• Organism: Pseudomonas aeruginosa strain PA1_NCHU

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: MON07_RS26400
• Protein ID: WP_003113526.1
• Coordinates: 5676650..5676928 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MON07_RS26405
• Protein ID: WP_003099268.1
• Coordinates: 5676940..5677245 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MON07_RS26380 (MON07_26380)	5672076..5673314	+	1239	WP_009316205.1	C69 family dipeptidase	-
MON07_RS26385 (MON07_26385)	5673376..5674023	+	648	WP_003095021.1	carbonate dehydratase	-
MON07_RS26390 (MON07_26390)	5674093..5676321	-	2229	WP_003099265.1	TonB-dependent receptor	-
MON07_RS26395 (MON07_26395)	5676469..5676597	+	129	Protein_5227	integrase	-
MON07_RS26400 (MON07_26400)	5676650..5676928	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MON07_RS26405 (MON07_26405)	5676940..5677245	+	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
MON07_RS26415 (MON07_26415)	5677642..5678742	-	1101	WP_003099270.1	redox-regulated ATPase YchF	-
MON07_RS26420 (MON07_26420)	5678783..5679367	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
MON07_RS26425 (MON07_26425)	5679409..5680023	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
MON07_RS26430 (MON07_26430)	5680140..5681081	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
MON07_RS26440 (MON07_26440)	5681248..5682096	-	849	WP_033969886.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T238441 WP_003113526.1 NZ_CP093395:5676650-5676928 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T238441 NZ_LR890344:55017-55499 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT238441 WP_003099268.1 NZ_CP093395:5676940-5677245 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT238441 NZ_LR890344:54763-55029 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: V6ALY3