Detailed information of TA system TA238428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2111183..2111745
• Replicon: chromosome
• Accession: NZ_CP093392
• Organism: Salmonella enterica strain P201982

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5U2RHV4
• Locus tag: MOV21_RS10640
• Protein ID: WP_071686223.1
• Coordinates: 2111467..2111745 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MOV21_RS10635
• Protein ID: WP_001240607.1
• Coordinates: 2111183..2111467 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOV21_RS10610 (2106851)	2106851..2107573	-	723	WP_048591017.1	SPI-1 type III secretion system guanine nucleotide exchange factor SopE	-
MOV21_RS10615 (2107783)	2107783..2107991	+	209	Protein_2083	helix-turn-helix domain-containing protein	-
MOV21_RS10620 (2108123)	2108123..2108824	-	702	WP_086126161.1	DUF1076 domain-containing protein	-
MOV21_RS10625 (2109648)	2109648..2110043	-	396	Protein_2085	tyrosine-type recombinase/integrase	-
MOV21_RS10630 (2110686)	2110686..2111024	+	339	WP_114050305.1	T3SS effector NleG family protein	-
MOV21_RS10635 (2111183)	2111183..2111467	-	285	WP_001240607.1	HigA family addiction module antitoxin	Antitoxin
MOV21_RS10640 (2111467)	2111467..2111745	-	279	WP_071686223.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MOV21_RS10645 (2111997)	2111997..2112257	+	261	WP_002953402.1	hypothetical protein	-
MOV21_RS10650 (2112328)	2112328..2113011	-	684	WP_238728147.1	PipA/GogA/GtgA family type III secretion system effector	-
MOV21_RS10655 (2113530)	2113530..2113988	+	459	WP_000502123.1	IS200/IS605-like element IS200F family transposase	-
MOV21_RS10660 (2114148)	2114148..2114978	+	831	WP_227012499.1	hypothetical protein	-
MOV21_RS10665 (2114981)	2114981..2115512	+	532	Protein_2093	tail fiber assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	sseI/srfH / sspH2 / sopE2 / sspH2 / sspH1 / sspH2	2001466..2130415	128949
	-	flank	IS/Tn	-	-	2113743..2113988	245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-63)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10751.24 Da
• Isoelectric Point: 9.4011

>T238428 WP_071686223.1 NZ_CP093392:c2111745-2111467 [Salmonella enterica]
MIKSFRHKGLKKFYQKGDSSGLEQSLVPRLKNRLSMLDAAKTIDFLNQQGYDLHELKGDRAETWSIKVSGNWRLTFIFEN
GDVYVLNLEDYH

Nucleotide

• Length: 279 bp

>T238428 NZ_LR890336:20212-20592 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCTGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10187.74 Da
• Isoelectric Point: 5.6051

>AT238428 WP_001240607.1 NZ_CP093392:c2111467-2111183 [Salmonella enterica]
MAMFDPAYPGEIIAEILEDQGVSLRQFAVAMNIAPSTASRILSGKVSITPEMAVKLAIVLGGDAESWLNLQNNYSLSQAR
KTVDITHVHRLSFA

Nucleotide

• Length: 285 bp

>AT238428 NZ_LR890336:19991-20212 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCACGCGGCAACCTTAGCGAAGTGCTCAATAATGTTGAAGCAGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTTGAAACCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A5U2RHV4