Detailed information of TA system TA238346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1329762..1330395
• Replicon: chromosome
• Accession: NZ_CP093380
• Organism: Rhodococcus opacus strain S8

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A3A9TJ70
• Locus tag: MOO23_RS06160
• Protein ID: WP_054248704.1
• Coordinates: 1330114..1330395 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MOO23_RS06155
• Protein ID: WP_007297375.1
• Coordinates: 1329762..1330055 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOO23_RS06130 (MOO23_06130)	1325187..1326308	-	1122	WP_242323095.1	sulfatase-like hydrolase/transferase	-
MOO23_RS06135 (MOO23_06135)	1326560..1327153	-	594	WP_242323096.1	helix-turn-helix domain-containing protein	-
MOO23_RS06140 (MOO23_06140)	1327427..1327783	-	357	WP_242323097.1	sulfatase-like hydrolase/transferase	-
MOO23_RS06145 (MOO23_06145)	1328092..1328400	-	309	WP_205627428.1	hypothetical protein	-
MOO23_RS06150 (MOO23_06150)	1328940..1329540	-	601	Protein_1230	recombinase family protein	-
MOO23_RS06155 (MOO23_06155)	1329762..1330055	-	294	WP_007297375.1	HigA family addiction module antitoxin	Antitoxin
MOO23_RS06160 (MOO23_06160)	1330114..1330395	-	282	WP_054248704.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MOO23_RS41150	1330638..1330769	-	132	WP_009480666.1	hypothetical protein	-
MOO23_RS06165 (MOO23_06165)	1331058..1331546	+	489	WP_242323098.1	hypothetical protein	-
MOO23_RS06170 (MOO23_06170)	1331748..1332623	-	876	WP_242323099.1	pentapeptide repeat-containing protein	-
MOO23_RS06175 (MOO23_06175)	1332850..1335204	+	2355	WP_242323100.1	ATP-binding cassette domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-81)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11006.51 Da
• Isoelectric Point: 8.9336

>T238346 WP_054248704.1 NZ_CP093380:c1330395-1330114 [Rhodococcus opacus]
VIESFADKETERVFHRQFSKKLPQDIQRIARRRLLLIEAATDVNDLRVPPGNRLEKLSGDRLGQYSVRINDQYRICFIWR
ATAAYDVEITDYH

Nucleotide

• Length: 282 bp

>T238346 NZ_LR890317:4104656-4105024 [Klebsiella pneumoniae]
ATGAAAAATCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCATCTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGTGATGAAGCCGTTATACAGGAACATATTG
ATGCCGGTATCACTCTGGCTAATGCTGTTAACTTTCTGGTGGAGAAGTATGAGTTAGTCCGTATCGATCGCTGTGGATTT
TCCTCACAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCATGCCAGGAAAGCGTGCGGGCTGATGAGCCGGTA
TTCTTACAGGGAGGTAAGTAATATTGTTCTTAGCCGATCACGGATATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10745.39 Da
• Isoelectric Point: 7.0123

>AT238346 WP_007297375.1 NZ_CP093380:c1330055-1329762 [Rhodococcus opacus]
MTTDLSPIHPGEILREDFLEPFGITQNRLATIIGVPPRRINEIVLGKRSITADTALRLAKAFGNSAQFWLNIQDRYELDL
AADKIGDALAKITRLSA

Nucleotide

• Length: 294 bp

>AT238346 NZ_LR890317:4104318-4104635 [Klebsiella pneumoniae]
ATGAGCAACCCCACATGGGGCCTGCTGCGGGATATCACGCCGTGCCTAGGAGCACGTCTGGTGCAGGAGGGCAACCGGCT
GTACTATCTGGTTGACCGAGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAATCCTGAAGTTGGATGTCGTATTTCCAC
ATTTTATCAGTCAGATGGAATCGATGCTGACCATTGGTGAAATGAATCCCCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATACATCGCCATTTATCTCACTCAACGCTAA

Structures

Toxin

• AlphaFold DB: A0A3A9TJ70