Detailed information of TA system TA238318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4690427..4691029
• Replicon: chromosome
• Accession: NZ_CP093373
• Organism: Salmonella enterica subsp. enterica serovar Infantis strain 423_13

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: MNU28_RS22745
• Protein ID: WP_001159630.1
• Coordinates: 4690427..4690738 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MNU28_RS22750
• Protein ID: WP_000362050.1
• Coordinates: 4690739..4691029 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNU28_RS22710 (4685540)	4685540..4686139	+	600	WP_000965698.1	glucose-1-phosphatase	-
MNU28_RS22715 (4686133)	4686133..4687005	+	873	WP_000921423.1	virulence factor BrkB family protein	-
MNU28_RS22720 (4687002)	4687002..4687439	+	438	WP_023993693.1	D-aminoacyl-tRNA deacylase	-
MNU28_RS22725 (4687484)	4687484..4688425	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
MNU28_RS22730 (4688440)	4688440..4688886	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
MNU28_RS22735 (4688883)	4688883..4689194	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
MNU28_RS22740 (4689280)	4689280..4690209	-	930	WP_021294279.1	alpha/beta hydrolase	-
MNU28_RS22745 (4690427)	4690427..4690738	+	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
MNU28_RS22750 (4690739)	4690739..4691029	+	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
MNU28_RS22755 (4691076)	4691076..4692005	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
MNU28_RS22760 (4692002)	4692002..4692637	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
MNU28_RS22765 (4692634)	4692634..4693536	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T238318 WP_001159630.1 NZ_CP093373:4690427-4690738 [Salmonella enterica subsp. enterica serovar Infantis]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T238318 NZ_LR890303:c9285-9037 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT238318 WP_000362050.1 NZ_CP093373:4690739-4691029 [Salmonella enterica subsp. enterica serovar Infantis]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT238318 NZ_LR890303:c9040-8693 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGGAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCACTGAACGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A0R9NS64