Detailed information of TA system TA238298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4489996..4490598
• Replicon: chromosome
• Accession: NZ_CP093369
• Organism: Escherichia coli strain EV36 mutant 5a

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: L0Q35_RS21640
• Protein ID: WP_000897302.1
• Coordinates: 4490287..4490598 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L0Q35_RS21635
• Protein ID: WP_000356397.1
• Coordinates: 4489996..4490286 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0Q35_RS21605 (4486069)	4486069..4486971	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
L0Q35_RS21610 (4486968)	4486968..4487603	+	636	WP_025857205.1	formate dehydrogenase cytochrome b556 subunit	-
L0Q35_RS21615 (4487600)	4487600..4488529	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
L0Q35_RS21620 (4488745)	4488745..4488963	-	219	WP_001314326.1	CopG family transcriptional regulator	-
L0Q35_RS21625 (4489359)	4489359..4489637	-	279	WP_001296612.1	hypothetical protein	-
L0Q35_RS21630 (4489699)	4489699..4489911	-	213	WP_000197774.1	hypothetical protein	-
L0Q35_RS21635 (4489996)	4489996..4490286	-	291	WP_000356397.1	NadS family protein	Antitoxin
L0Q35_RS21640 (4490287)	4490287..4490598	-	312	WP_000897302.1	hypothetical protein	Toxin
L0Q35_RS21645 (4490827)	4490827..4491735	+	909	WP_001331553.1	alpha/beta hydrolase	-
L0Q35_RS21650 (4491799)	4491799..4492740	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
L0Q35_RS21655 (4492785)	4492785..4493222	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
L0Q35_RS21660 (4493219)	4493219..4494091	-	873	WP_025857204.1	virulence factor BrkB family protein	-
L0Q35_RS21665 (4494085)	4494085..4494684	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T238298 WP_000897302.1 NZ_CP093369:c4490598-4490287 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T238298 NZ_LR890299:2766447-2766824 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTGCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGATTC
AGCGCAGGAACTTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT238298 WP_000356397.1 NZ_CP093369:c4490286-4489996 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT238298 NZ_LR890299:2765989-2766357 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCACCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: S1P416