Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4205522..4206321 Replicon chromosome
Accession NZ_CP093369
Organism Escherichia coli strain EV36 mutant 5a

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag L0Q35_RS20300 Protein ID WP_000347273.1
Coordinates 4205857..4206321 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag L0Q35_RS20295 Protein ID WP_001307405.1
Coordinates 4205522..4205857 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0Q35_RS20280 (4201307) 4201307..4202077 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
L0Q35_RS20285 (4202093) 4202093..4203427 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
L0Q35_RS20290 (4203802) 4203802..4205373 + 1572 WP_001273753.1 galactarate dehydratase -
L0Q35_RS20295 (4205522) 4205522..4205857 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L0Q35_RS20300 (4205857) 4205857..4206321 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L0Q35_RS20305 (4206376) 4206376..4207185 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L0Q35_RS20310 (4207434) 4207434..4208714 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
L0Q35_RS20315 (4208737) 4208737..4209210 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
L0Q35_RS20320 (4209221) 4209221..4209592 + 372 Protein_3992 PTS sugar transporter subunit IIC -
L0Q35_RS20325 (4209588) 4209588..4210145 + 558 Protein_3993 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4196374..4217305 20931


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T238297 WP_000347273.1 NZ_CP093369:4205857-4206321 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T238297 NZ_LR890299:c2615504-2615154 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT238297 WP_001307405.1 NZ_CP093369:4205522-4205857 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT238297 NZ_LR890299:c2615749-2615498 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References