Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4162248..4162975 Replicon chromosome
Accession NZ_CP093369
Organism Escherichia coli strain EV36 mutant 5a

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag L0Q35_RS20075 Protein ID WP_000550189.1
Coordinates 4162661..4162975 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag L0Q35_RS20070 Protein ID WP_000560266.1
Coordinates 4162248..4162664 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0Q35_RS20060 (4157408) 4157408..4159759 + 2352 WP_000695487.1 alpha-glucosidase -
L0Q35_RS20065 (4160185) 4160185..4162203 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
L0Q35_RS20070 (4162248) 4162248..4162664 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
L0Q35_RS20075 (4162661) 4162661..4162975 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
L0Q35_RS20080 (4163259) 4163259..4164395 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
L0Q35_RS20085 (4164480) 4164480..4164983 + 504 WP_001333820.1 M48 family metallopeptidase -
L0Q35_RS20090 (4165060) 4165060..4165752 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
L0Q35_RS20095 (4165831) 4165831..4166817 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T238296 WP_000550189.1 NZ_CP093369:c4162975-4162661 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T238296 NZ_LR890299:c2603782-2603492 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT238296 WP_000560266.1 NZ_CP093369:c4162664-4162248 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT238296 NZ_LR890299:c2604014-2603772 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References