238290

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2911152..2911654 Replicon chromosome
Accession NZ_CP093369
Organism Escherichia coli strain EV36 mutant 5a

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag L0Q35_RS14225 Protein ID WP_000767829.1
Coordinates 2911152..2911406 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag L0Q35_RS14230 Protein ID WP_001259255.1
Coordinates 2911403..2911654 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0Q35_RS14195 (2906167) 2906167..2906394 - 228 WP_000234896.1 sulfurtransferase TusA -
L0Q35_RS14200 (2906408) 2906408..2907466 - 1059 WP_000492339.1 thiosulfate utilization transporter TsuA/YeeE -
L0Q35_RS14205 (2907645) 2907645..2909003 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
L0Q35_RS14210 (2908993) 2908993..2909055 - 63 WP_010723108.1 membrane protein YoeI -
L0Q35_RS14215 (2909270) 2909270..2910199 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
L0Q35_RS14220 (2910245) 2910245..2911069 - 825 WP_000754737.1 SDR family oxidoreductase -
L0Q35_RS14225 (2911152) 2911152..2911406 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
L0Q35_RS14230 (2911403) 2911403..2911654 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
L0Q35_RS14235 (2911937) 2911937..2911987 + 51 WP_001364200.1 his operon leader peptide -
L0Q35_RS14240 (2912133) 2912133..2913032 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
L0Q35_RS14245 (2913038) 2913038..2914342 + 1305 WP_000009594.1 histidinol dehydrogenase -
L0Q35_RS14250 (2914339) 2914339..2915409 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
L0Q35_RS14255 (2915409) 2915409..2916476 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T238290 WP_000767829.1 NZ_CP093369:c2911406-2911152 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T238290 NZ_LR890299:1033423-1033530 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACTTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT238290 WP_001259255.1 NZ_CP093369:c2911654-2911403 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT238290 NZ_LR890299:c1033373-1033310 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References