Detailed information of TA system TA238269

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4521018..4521620
• Replicon: chromosome
• Accession: NZ_CP093368
• Organism: Escherichia coli strain EV36 mutant 1a

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: L0S95_RS21835
• Protein ID: WP_000897302.1
• Coordinates: 4521309..4521620 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: L0S95_RS21830
• Protein ID: WP_000356397.1
• Coordinates: 4521018..4521308 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0S95_RS21800 (4517091)	4517091..4517993	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
L0S95_RS21805 (4517990)	4517990..4518625	+	636	WP_025857205.1	formate dehydrogenase cytochrome b556 subunit	-
L0S95_RS21810 (4518622)	4518622..4519551	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
L0S95_RS21815 (4519767)	4519767..4519985	-	219	WP_001314326.1	CopG family transcriptional regulator	-
L0S95_RS21820 (4520381)	4520381..4520659	-	279	WP_001296612.1	hypothetical protein	-
L0S95_RS21825 (4520721)	4520721..4520933	-	213	WP_000197774.1	hypothetical protein	-
L0S95_RS21830 (4521018)	4521018..4521308	-	291	WP_000356397.1	NadS family protein	Antitoxin
L0S95_RS21835 (4521309)	4521309..4521620	-	312	WP_000897302.1	hypothetical protein	Toxin
L0S95_RS21840 (4521849)	4521849..4522757	+	909	WP_001331553.1	alpha/beta hydrolase	-
L0S95_RS21845 (4522821)	4522821..4523762	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
L0S95_RS21850 (4523807)	4523807..4524244	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
L0S95_RS21855 (4524241)	4524241..4525113	-	873	WP_025857204.1	virulence factor BrkB family protein	-
L0S95_RS21860 (4525107)	4525107..4525706	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T238269 WP_000897302.1 NZ_CP093368:c4521620-4521309 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T238269 NZ_LR890290:2039-2320 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCTGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGTCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT238269 WP_000356397.1 NZ_CP093368:c4521308-4521018 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT238269 NZ_LR890290:1773-2042 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: S1P416