Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4236544..4237343 Replicon chromosome
Accession NZ_CP093368
Organism Escherichia coli strain EV36 mutant 1a

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag L0S95_RS20495 Protein ID WP_000347273.1
Coordinates 4236879..4237343 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag L0S95_RS20490 Protein ID WP_001307405.1
Coordinates 4236544..4236879 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0S95_RS20475 (4232329) 4232329..4233099 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
L0S95_RS20480 (4233115) 4233115..4234449 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
L0S95_RS20485 (4234824) 4234824..4236395 + 1572 WP_001273753.1 galactarate dehydratase -
L0S95_RS20490 (4236544) 4236544..4236879 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
L0S95_RS20495 (4236879) 4236879..4237343 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
L0S95_RS20500 (4237398) 4237398..4238207 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
L0S95_RS20505 (4238456) 4238456..4239736 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
L0S95_RS20510 (4239759) 4239759..4240232 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
L0S95_RS20515 (4240243) 4240243..4240614 + 372 Protein_4029 PTS sugar transporter subunit IIC -
L0S95_RS20520 (4240610) 4240610..4241167 + 558 Protein_4030 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4227396..4248327 20931


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T238268 WP_000347273.1 NZ_CP093368:4236879-4237343 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T238268 NZ_LR890289:150748-151164 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT238268 WP_001307405.1 NZ_CP093368:4236544-4236879 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT238268 NZ_LR890289:150521-150751 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References