Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4193270..4193997 Replicon chromosome
Accession NZ_CP093368
Organism Escherichia coli strain EV36 mutant 1a

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag L0S95_RS20270 Protein ID WP_000550189.1
Coordinates 4193683..4193997 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag L0S95_RS20265 Protein ID WP_000560266.1
Coordinates 4193270..4193686 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L0S95_RS20255 (4188430) 4188430..4190781 + 2352 WP_000695487.1 alpha-glucosidase -
L0S95_RS20260 (4191207) 4191207..4193225 + 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
L0S95_RS20265 (4193270) 4193270..4193686 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
L0S95_RS20270 (4193683) 4193683..4193997 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
L0S95_RS20275 (4194281) 4194281..4195417 - 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
L0S95_RS20280 (4195502) 4195502..4196005 + 504 WP_001333820.1 M48 family metallopeptidase -
L0S95_RS20285 (4196082) 4196082..4196774 + 693 WP_000942548.1 vancomycin high temperature exclusion protein -
L0S95_RS20290 (4196853) 4196853..4197839 + 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T238267 WP_000550189.1 NZ_CP093368:c4193997-4193683 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T238267 NZ_LR890289:c149741-149436 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT238267 WP_000560266.1 NZ_CP093368:c4193686-4193270 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT238267 NZ_LR890289:c149961-149743 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References