Detailed information of TA system TA238246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1144018..1144712
• Replicon: chromosome
• Accession: NZ_CP093368
• Organism: Escherichia coli strain EV36 mutant 1a

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: L0S95_RS05480
• Protein ID: WP_001263489.1
• Coordinates: 1144314..1144712 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: L0S95_RS05475
• Protein ID: WP_000554758.1
• Coordinates: 1144018..1144311 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L0S95_RS05455 (1139650)	1139650..1140147	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
L0S95_RS05460 (1140371)	1140371..1142083	-	1713	Protein_1073	flagellar biosynthesis protein FlhA	-
L0S95_RS05465 (1142055)	1142055..1142840	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
L0S95_RS05470 (1142911)	1142911..1143966	+	1056	WP_001226164.1	DNA polymerase IV	-
L0S95_RS05475 (1144018)	1144018..1144311	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
L0S95_RS05480 (1144314)	1144314..1144712	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
L0S95_RS05485 (1144722)	1144722..1145174	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
L0S95_RS05490 (1145492)	1145492..1145698	+	207	Protein_1079	RtcB family protein	-
L0S95_RS05495 (1145694)	1145694..1146215	+	522	Protein_1080	peptide chain release factor H	-
L0S95_RS05500 (1146272)	1146272..1147729	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
L0S95_RS05505 (1147990)	1147990..1148448	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (1149044)	1149044..1149124	+	81	NuclAT_4	-	-
- (1149044)	1149044..1149124	+	81	NuclAT_4	-	-
- (1149044)	1149044..1149124	+	81	NuclAT_4	-	-
- (1149044)	1149044..1149124	+	81	NuclAT_4	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T238246 WP_001263489.1 NZ_CP093368:1144314-1144712 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T238246 NZ_LR890288:836278-836685 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT238246 WP_000554758.1 NZ_CP093368:1144018-1144311 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT238246 NZ_LR890288:836031-836297 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3