Detailed information of TA system TA238228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5585754..5586349
• Replicon: chromosome
• Accession: NZ_CP093357
• Organism: Pseudomonas aeruginosa strain E131

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: MN179_RS26095
• Protein ID: WP_003117425.1
• Coordinates: 5586071..5586349 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MN179_RS26090
• Protein ID: WP_003113527.1
• Coordinates: 5585754..5586059 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MN179_RS26055 (MN179_26055)	5580893..5581741	+	849	WP_023114865.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
MN179_RS26065 (MN179_26065)	5581908..5582849	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
MN179_RS26070 (MN179_26070)	5582966..5583580	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
MN179_RS26075 (MN179_26075)	5583622..5584206	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
MN179_RS26080 (MN179_26080)	5584247..5585347	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
MN179_RS26090 (MN179_26090)	5585754..5586059	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
MN179_RS26095 (MN179_26095)	5586071..5586349	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MN179_RS26100 (MN179_26100)	5586402..5586530	-	129	Protein_5171	integrase	-
MN179_RS26105 (MN179_26105)	5586678..5588906	+	2229	WP_003113525.1	TonB-dependent receptor	-
MN179_RS26110 (MN179_26110)	5588976..5589623	-	648	WP_003095021.1	carbonate dehydratase	-
MN179_RS26115 (MN179_26115)	5589685..5590923	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T238228 WP_003117425.1 NZ_CP093357:c5586349-5586071 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T238228 NZ_LR890278:46498-46821 [Klebsiella pneumoniae]
ATGAACTATCTGGAGTTTGTTGAAACCAGCGCTTTCAGTGGACTGCGTAAGGAACTCATGGATGATGATGAATTCCGCGA
ACTGCAGACCTATTTACTGGAAGCCCATGATCGGGGTGACACCATCAGTCATACCGGTGGATGCAGGAAGATTCGCTGGG
GTCGCCCGGGGATGGGTAAGCGAGGTGGCGTCAGGGTGATTTACTACGTTCGTCTTGCCAGCGGCAGACTGTATCTGCTG
CTGATATACCCGAAGAATGCGAAAGATGAGCTCAGCGAGAAAGAAAAAGCCGTTATGAAGGCATTAACACAGCAGCTGAA
GTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT238228 WP_003113527.1 NZ_CP093357:c5586059-5585754 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT238228 NZ_LR890278:46870-47172 [Klebsiella pneumoniae]
ATGGAAAAGAAACTTTTTGATGCCCTGATCAGCAGCATGGAGGAAATGGTGGCCATTGAAAATGGCCAGATCCAGCCCGC
TGCAGAACAGGTTCACCGCCATACACTGCCGGATGTGAAAGCCATTCGTAAACATGCCGGCATGAAACAGGCTGAATTTG
CAGAAGTGGTGGGGGCCAGCGTTGACCTTGTTCGCAGCTGGGAACAGCAACGGCGTGTTCCGTCGGGTATTGCGCTGAAA
ATGCTCTGCCTCATCGAGCGTGAGCCTGCACTGATTAATACGTTGCGTGGTATTCACGTCTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5