Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 5821678..5822273 Replicon chromosome
Accession NZ_CP093356
Organism Pseudomonas aeruginosa strain E125

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag MN178_RS27440 Protein ID WP_003113526.1
Coordinates 5821995..5822273 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MN178_RS27435 Protein ID WP_003113527.1
Coordinates 5821678..5821983 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MN178_RS27415 (MN178_27415) 5817152..5817487 + 336 WP_031633682.1 hypothetical protein -
MN178_RS27420 (MN178_27420) 5818019..5819785 - 1767 WP_023087829.1 anti-phage dCTP deaminase -
MN178_RS27425 (MN178_27425) 5820042..5820704 - 663 WP_023087830.1 restriction endonuclease -
MN178_RS27430 (MN178_27430) 5820928..5821341 - 414 WP_049231965.1 hypothetical protein -
MN178_RS27435 (MN178_27435) 5821678..5821983 - 306 WP_003113527.1 HigA family addiction module antitoxin Antitoxin
MN178_RS27440 (MN178_27440) 5821995..5822273 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MN178_RS27445 (MN178_27445) 5822326..5822454 - 129 Protein_5443 integrase -
MN178_RS27450 (MN178_27450) 5822602..5824830 + 2229 WP_003113525.1 TonB-dependent receptor -
MN178_RS27455 (MN178_27455) 5824900..5825547 - 648 WP_003095021.1 carbonate dehydratase -
MN178_RS27460 (MN178_27460) 5825609..5826847 - 1239 WP_003113524.1 dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T238220 WP_003113526.1 NZ_CP093356:c5822273-5821995 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T238220 NZ_LR890277:1865088-1865190 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11247.81 Da        Isoelectric Point: 5.0747

>AT238220 WP_003113527.1 NZ_CP093356:c5821983-5821678 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Download         Length: 306 bp

>AT238220 NZ_LR890277:c1865197-1865052 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure

References