Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2798851..2799626 Replicon chromosome
Accession NZ_CP093356
Organism Pseudomonas aeruginosa strain E125

Toxin (Protein)


Gene name yhaV Uniprot ID V6ADY6
Locus tag MN178_RS13285 Protein ID WP_009518525.1
Coordinates 2799168..2799626 (+) Length 153 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A7M2ZSF3
Locus tag MN178_RS13280 Protein ID WP_023087286.1
Coordinates 2798851..2799168 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MN178_RS13250 (MN178_13250) 2794175..2794825 - 651 WP_023087281.1 isoprenylcysteine carboxylmethyltransferase family protein -
MN178_RS13255 (MN178_13255) 2794822..2795061 - 240 WP_023087282.1 DUF2933 domain-containing protein -
MN178_RS13260 (MN178_13260) 2795073..2795474 - 402 WP_023087283.1 hypothetical protein -
MN178_RS13265 (MN178_13265) 2795629..2796063 - 435 WP_078452152.1 hypothetical protein -
MN178_RS13270 (MN178_13270) 2796136..2796630 + 495 WP_031633853.1 MerR family DNA-binding protein -
MN178_RS13275 (MN178_13275) 2796686..2798551 - 1866 WP_023087285.1 MobH family relaxase -
MN178_RS13280 (MN178_13280) 2798851..2799168 + 318 WP_023087286.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
MN178_RS13285 (MN178_13285) 2799168..2799626 + 459 WP_009518525.1 type II toxin-antitoxin system YhaV family toxin Toxin
MN178_RS13290 (MN178_13290) 2799653..2800012 + 360 WP_023087287.1 DUF3742 family protein -
MN178_RS13295 (MN178_13295) 2800019..2801542 - 1524 WP_023087288.1 conjugal transfer protein TraG N-terminal domain-containing protein -
MN178_RS13300 (MN178_13300) 2801556..2801915 - 360 WP_023087289.1 hypothetical protein -
MN178_RS13305 (MN178_13305) 2801912..2803306 - 1395 WP_023087290.1 integrating conjugative element protein -
MN178_RS13310 (MN178_13310) 2803316..2804263 - 948 WP_023087291.1 TIGR03756 family integrating conjugative element protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2745564..2860771 115207


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(8-144)

Antitoxin

PrlF antitoxin

(8-100)


Sequences

Toxin        


Download         Length: 153 a.a.        Molecular weight: 17660.02 Da        Isoelectric Point: 10.1848

>T238215 WP_009518525.1 NZ_CP093356:2799168-2799626 [Pseudomonas aeruginosa]
MQRHGWTLLFHDCVIEQLQKLHAAARRAQENDPAGFESNANVKLFRALSQLMLDVVPGDPARDEYRQGNTLGPAHRHWRR
AKIGRRFRLFFRYDSKAKVIVYAWVNDEQTLRSSGSKSDPYVVFEKMLGRGNPPDDWHALIQASKQDWSKLE

Download         Length: 459 bp

>T238215 NZ_LR890273:c42991-42611 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCTGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAACTATATGGTTCCAATATTTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11156.69 Da        Isoelectric Point: 4.5685

>AT238215 WP_023087286.1 NZ_CP093356:2798851-2799168 [Pseudomonas aeruginosa]
MPAIHEVATLTSKGQITLPKPIRQALGVDAGGKLAFDLRGSEVVVTRVDAEHEDPAIGAFLSLLARDIEAGRNLQGLPED
LARAMLEHAGHGVNLDEEIDGKVEL

Download         Length: 318 bp

>AT238215 NZ_LR890273:c43212-42991 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCACGCGGCAACCTTAGCGAAGTGCTCAATAATGTTGAAGCAGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ADY6

Antitoxin

Source ID Structure
AlphaFold DB A0A7M2ZSF3

References