Detailed information of TA system TA238212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5599508..5600103
• Replicon: chromosome
• Accession: NZ_CP093355
• Organism: Pseudomonas aeruginosa strain E104

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: LRN73_RS26255
• Protein ID: WP_003117425.1
• Coordinates: 5599825..5600103 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LRN73_RS26250
• Protein ID: WP_003113527.1
• Coordinates: 5599508..5599813 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LRN73_RS26215 (LRN73_26215)	5594647..5595495	+	849	WP_023114865.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
LRN73_RS26225 (LRN73_26225)	5595662..5596603	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
LRN73_RS26230 (LRN73_26230)	5596720..5597334	+	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
LRN73_RS26235 (LRN73_26235)	5597376..5597960	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
LRN73_RS26240 (LRN73_26240)	5598001..5599101	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
LRN73_RS26250 (LRN73_26250)	5599508..5599813	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
LRN73_RS26255 (LRN73_26255)	5599825..5600103	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LRN73_RS26260 (LRN73_26260)	5600156..5600284	-	129	Protein_5204	integrase	-
LRN73_RS26265 (LRN73_26265)	5600432..5602660	+	2229	WP_003113525.1	TonB-dependent receptor	-
LRN73_RS26270 (LRN73_26270)	5602730..5603377	-	648	WP_003095021.1	carbonate dehydratase	-
LRN73_RS26275 (LRN73_26275)	5603439..5604677	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T238212 WP_003117425.1 NZ_CP093355:c5600103-5599825 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T238212 NZ_LR890272:2269-2544 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGACCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT238212 WP_003113527.1 NZ_CP093355:c5599813-5599508 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT238212 NZ_LR890272:1985-2269 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5