Detailed information of TA system TA238205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5789261..5789856
• Replicon: chromosome
• Accession: NZ_CP093354
• Organism: Pseudomonas aeruginosa strain E113

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: MN130_RS27025
• Protein ID: WP_003113526.1
• Coordinates: 5789578..5789856 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MN130_RS27020
• Protein ID: WP_003099268.1
• Coordinates: 5789261..5789566 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MN130_RS26985 (MN130_26985)	5784369..5784659	-	291	WP_023088865.1	DUF5447 family protein	-
MN130_RS26990 (MN130_26990)	5784663..5784845	-	183	WP_124129801.1	hypothetical protein	-
MN130_RS26995 (MN130_26995)	5784835..5785107	-	273	WP_004352675.1	hypothetical protein	-
MN130_RS27000 (MN130_27000)	5785554..5785889	+	336	WP_031756230.1	hypothetical protein	-
MN130_RS27005 (MN130_27005)	5785944..5787662	-	1719	WP_228772291.1	DUF2326 domain-containing protein	-
MN130_RS27010 (MN130_27010)	5787848..5788396	-	549	WP_031756228.1	hypothetical protein	-
MN130_RS27015 (MN130_27015)	5788404..5788658	-	255	WP_071575691.1	hypothetical protein	-
MN130_RS27020 (MN130_27020)	5789261..5789566	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
MN130_RS27025 (MN130_27025)	5789578..5789856	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MN130_RS27030 (MN130_27030)	5789909..5790037	-	129	Protein_5354	integrase	-
MN130_RS27035 (MN130_27035)	5790185..5792413	+	2229	WP_003099265.1	TonB-dependent receptor	-
MN130_RS27040 (MN130_27040)	5792483..5793130	-	648	WP_003095021.1	carbonate dehydratase	-
MN130_RS27045 (MN130_27045)	5793192..5794430	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T238205 WP_003113526.1 NZ_CP093354:c5789856-5789578 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T238205 NZ_LR890270:c4149624-4149406 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT238205 WP_003099268.1 NZ_CP093354:c5789566-5789261 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT238205 NZ_LR890270:c4150024-4149650 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: V6ALY3