Detailed information of TA system TA238171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 2655107..2656461
• Replicon: chromosome
• Accession: NZ_CP093332
• Organism: Buttiauxella ferragutiae strain H4-C11

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: MNO13_RS12285
• Protein ID: WP_241938787.1
• Coordinates: 2655107..2656144 (-)
• Length: 346 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A3G2I9X5
• Locus tag: MNO13_RS12290
• Protein ID: WP_064546608.1
• Coordinates: 2656147..2656461 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNO13_RS12250 (MNO13_12250)	2650537..2650725	-	189	WP_064546594.1	YegP family protein	-
MNO13_RS12255 (MNO13_12255)	2650892..2651065	+	174	WP_064546596.1	GhoT/OrtT family toxin	-
MNO13_RS12260 (MNO13_12260)	2651106..2651423	+	318	WP_064546598.1	N(4)-acetylcytidine aminohydrolase	-
MNO13_RS12265 (MNO13_12265)	2651464..2651679	-	216	WP_064546600.1	YdcH family protein	-
MNO13_RS12270 (MNO13_12270)	2651950..2652711	+	762	WP_232885839.1	siderophore-interacting protein	-
MNO13_RS12275 (MNO13_12275)	2652722..2653306	+	585	WP_083963375.1	PadR family transcriptional regulator	-
MNO13_RS12280 (MNO13_12280)	2653393..2655057	+	1665	WP_241938786.1	methyl-accepting chemotaxis protein	-
MNO13_RS12285 (MNO13_12285)	2655107..2656144	-	1038	WP_241938787.1	HipA domain-containing protein	Toxin
MNO13_RS12290 (MNO13_12290)	2656147..2656461	-	315	WP_064546608.1	HipA N-terminal domain-containing protein	Antitoxin
MNO13_RS12295 (MNO13_12295)	2656446..2656769	-	324	WP_241938788.1	helix-turn-helix domain-containing protein	-
MNO13_RS12300 (MNO13_12300)	2657083..2658111	+	1029	WP_241938789.1	MsnO8 family LLM class oxidoreductase	-
MNO13_RS12305 (MNO13_12305)	2658122..2659243	+	1122	WP_064546614.1	amidohydrolase	-
MNO13_RS12310 (MNO13_12310)	2659307..2660608	+	1302	Protein_2414	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-303)

Antitoxin

• HipA, N-terminal subdomain 1 (5-99)

Sequences

Toxin

Protein

• Length: 346 a.a.
• Molecular weight: 38880.55 Da
• Isoelectric Point: 6.4312

>T238171 WP_241938787.1 NZ_CP093332:c2656144-2655107 [Buttiauxella ferragutiae]
MRCHILLTPLLQAKEQESGYSSKGLKQLTGSGKVNPQLSFTRDDFIHELPKAQRGMSISGYQPKLQMVVRAGEFAVVEHQ
GEYILKPSPAEFPHLAENEHATMTLMARLGFDVPPHGLVSFAAVDNEAERELAFVIRRFDRDIQTGAAIHQEQLDAAMNV
GEKYGKTGSDGKQYVSYQQLAEFLTQHVNSNIAFKIDLFRRIAYAYLLGNNDMHLRNFGLIHPRIGEPTLSPVYDFVSVA
PYPAIFSSGFMALPLLRCEEGDRELAPGFKTAYGEYLGMDFQALGLSMGMTEKLIVKLLNDIRKESRIVDAVYRDSFMPA
KDVEATLRCYHQRLDRLMRLDEAEL

Nucleotide

• Length: 1038 bp

>T238171 NZ_LR890245:c4634130-4633597 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12014.68 Da
• Isoelectric Point: 6.2228

>AT238171 WP_064546608.1 NZ_CP093332:c2656461-2656147 [Buttiauxella ferragutiae]
MRRRVEVWLYGEPVGELRQHDDGFEFSYRADYIGPPLSLSLPVRVGHFRSQTLPPFFASLAPEGWLKKRYSQLQQRDEHD
LLGMLIDNGKNLIGAVQLVSMQED

Nucleotide

• Length: 315 bp

>AT238171 NZ_LR890245:c4634407-4634141 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3G2I9X5