Detailed information of TA system TA238160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1736949..1737615
• Replicon: chromosome
• Accession: NZ_CP093332
• Organism: Buttiauxella ferragutiae strain H4-C11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MNO13_RS07855
• Protein ID: WP_241939720.1
• Coordinates: 1736949..1737296 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A3G2IKP2
• Locus tag: MNO13_RS07860
• Protein ID: WP_064545179.1
• Coordinates: 1737298..1737615 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNO13_RS07845 (MNO13_07845)	1732344..1735469	-	3126	WP_237930347.1	MdtB/MuxB family multidrug efflux RND transporter permease subunit	-
MNO13_RS07850 (MNO13_07850)	1735469..1736719	-	1251	WP_232886677.1	MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit	-
MNO13_RS07855 (MNO13_07855)	1736949..1737296	+	348	WP_241939720.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MNO13_RS07860 (MNO13_07860)	1737298..1737615	+	318	WP_064545179.1	helix-turn-helix transcriptional regulator	Antitoxin
MNO13_RS07865 (MNO13_07865)	1737647..1739005	-	1359	WP_064545181.1	molecular chaperone	-
MNO13_RS07870 (MNO13_07870)	1739104..1739955	+	852	WP_241939721.1	DNA-3-methyladenine glycosylase 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13856.01 Da
• Isoelectric Point: 9.9761

>T238160 WP_241939720.1 NZ_CP093332:1736949-1737296 [Buttiauxella ferragutiae]
MTWKILTTKRYEEWFDEQHNDLQEKMLAVMGHLRFWGPYLPRPYADTVNGSRFANMKELRVQHQGKPVRAFYIFDYRRNA
VLLCAGDKSKTKRFYESMITIADKEFIAWLKSQEK

Nucleotide

• Length: 348 bp

>T238160 NZ_LR890240:3972050-3972268 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11766.59 Da
• Isoelectric Point: 8.0036

>AT238160 WP_064545179.1 NZ_CP093332:1737298-1737615 [Buttiauxella ferragutiae]
MKNNPKGYSTLEELIARRSPESQQRIAEATQELILESCLNMIREQMGWSQQQLADAMGISQPAVTAIEKRGNEVKLGTLK
RYIEALGGKLSLQIEFPDGVKHLKI

Nucleotide

• Length: 318 bp

>AT238160 NZ_LR890240:3971649-3972023 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAACTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A3G2IKP2