Detailed information of TA system TA238155

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 59287..59795
• Replicon: plasmid p1
• Accession: NZ_CP093329
• Organism: Rahnella aceris strain S2-A69

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: H8P157
• Locus tag: MNO10_RS22060
• Protein ID: WP_013578404.1
• Coordinates: 59287..59553 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: H8P156
• Locus tag: MNO10_RS22065
• Protein ID: WP_013578405.1
• Coordinates: 59550..59795 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNO10_RS22050 (MNO10_22050)	54923..57022	+	2100	WP_013578365.1	peptidase domain-containing ABC transporter	-
MNO10_RS22055 (MNO10_22055)	57380..58966	-	1587	WP_013578364.1	sensor domain-containing diguanylate cyclase	-
MNO10_RS22060 (MNO10_22060)	59287..59553	-	267	WP_013578404.1	Txe/YoeB family addiction module toxin	Toxin
MNO10_RS22065 (MNO10_22065)	59550..59795	-	246	WP_013578405.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MNO10_RS22070 (MNO10_22070)	60105..61154	+	1050	WP_014683046.1	tyrosine-type recombinase/integrase	-
MNO10_RS22075 (MNO10_22075)	61246..61725	-	480	WP_014683045.1	GNAT family N-acetyltransferase	-
MNO10_RS22080 (MNO10_22080)	61722..61982	-	261	WP_200227549.1	DUF1778 domain-containing protein	-
MNO10_RS22085 (MNO10_22085)	62134..62475	+	342	WP_241917281.1	MFS transporter	-
MNO10_RS22090 (MNO10_22090)	62823..64283	+	1461	WP_014683042.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..123458	123458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10490.04 Da
• Isoelectric Point: 10.0392

>T238155 WP_013578404.1 NZ_CP093329:c59553-59287 [Rahnella aceris]
MKTRFTKRGAADYQYWQGSNAKIFERIRILIHAIHADPLGGIGKPERLRHYKNPALYSRRIDREHRLVYSMVNDELTIIA
CRYHYDDV

Nucleotide

• Length: 267 bp

>T238155 NZ_LR890240:349018-349365 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATTCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9027.06 Da
• Isoelectric Point: 4.1947

>AT238155 WP_013578405.1 NZ_CP093329:c59795-59550 [Rahnella aceris]
MHSLTFTDARKHFAETMQQVSDNAEPVRILRRDAPDVVLIDAAEYDAILETLYLFSNPANAAHINASLAELDRGDTVEIE
V

Nucleotide

• Length: 246 bp

>AT238155 NZ_LR890240:349365-349664 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: H8P157

Antitoxin

• AlphaFold DB: H8P156