Detailed information of TA system TA238089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13609..14266
• Replicon: plasmid pHUM3355_A
• Accession: NZ_CP093318
• Organism: Escherichia coli strain HUM3355

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: MLA23_RS22795
• Protein ID: WP_000270043.1
• Coordinates: 13916..14266 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MLA23_RS22790
• Protein ID: WP_000124640.1
• Coordinates: 13609..13911 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MLA23_RS22740 (MLA23_22720)	9234..9662	+	429	WP_000591074.1	hypothetical protein	-
MLA23_RS22745 (MLA23_22725)	9719..10078	+	360	WP_000422768.1	hypothetical protein	-
MLA23_RS22750 (MLA23_22730)	10078..10524	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
MLA23_RS22755 (MLA23_22735)	10521..11039	+	519	WP_000210756.1	nitrite reductase	-
MLA23_RS22760 (MLA23_22740)	11039..11269	+	231	WP_000972663.1	hypothetical protein	-
MLA23_RS22765 (MLA23_22745)	11256..12113	+	858	WP_001167032.1	hypothetical protein	-
MLA23_RS22770 (MLA23_22750)	12139..12327	+	189	WP_001270411.1	hypothetical protein	-
MLA23_RS22775 (MLA23_22755)	12344..12871	+	528	WP_001236377.1	thermonuclease family protein	-
MLA23_RS22780 (MLA23_22760)	12929..13201	+	273	WP_001043047.1	HU family DNA-binding protein	-
MLA23_RS22785 (MLA23_22765)	13289..13582	+	294	WP_001239997.1	chromosome segregation protein ParM	-
MLA23_RS22790 (MLA23_22770)	13609..13911	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
MLA23_RS22795 (MLA23_22775)	13916..14266	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MLA23_RS22800 (MLA23_22780)	14429..14977	+	549	WP_001061574.1	transcriptional regulator	-
MLA23_RS22805 (MLA23_22785)	15318..15512	+	195	WP_000343597.1	hypothetical protein	-
MLA23_RS22810 (MLA23_22790)	15523..15894	+	372	WP_000516916.1	hypothetical protein	-
MLA23_RS22815 (MLA23_22795)	15887..16357	+	471	WP_001281821.1	hypothetical protein	-
MLA23_RS22820 (MLA23_22800)	16372..16707	-	336	WP_000683476.1	hypothetical protein	-
MLA23_RS22825 (MLA23_22805)	16804..17292	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
MLA23_RS22830 (MLA23_22810)	17295..17792	+	498	WP_000062185.1	hypothetical protein	-
MLA23_RS22835 (MLA23_22815)	18007..18711	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
MLA23_RS22840 (MLA23_22820)	18776..18955	-	180	Protein_32	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ARR-3 / cmlA1 / blaOXA-10 / ant(3'')-Ia / qacE / sul1 / dfrA23 / qnrB1 / tet(A) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2	-	1..168684	168684

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T238089 WP_000270043.1 NZ_CP093318:c14266-13916 [Escherichia coli]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T238089 NZ_LR890204:781803-782213 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT238089 WP_000124640.1 NZ_CP093318:c13911-13609 [Escherichia coli]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT238089 NZ_LR890204:781556-781822 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9