Detailed information of TA system TA238085

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3677709..3678403
• Replicon: chromosome
• Accession: NZ_CP093317
• Organism: Escherichia coli strain HUM3355

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: MLA23_RS17795
• Protein ID: WP_001263493.1
• Coordinates: 3677709..3678107 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: MLA23_RS17800
• Protein ID: WP_000554757.1
• Coordinates: 3678110..3678403 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3673369)	3673369..3673449	-	81	NuclAT_11	-	-
- (3673369)	3673369..3673449	-	81	NuclAT_11	-	-
- (3673369)	3673369..3673449	-	81	NuclAT_11	-	-
- (3673369)	3673369..3673449	-	81	NuclAT_11	-	-
MLA23_RS17765 (3672709)	3672709..3673953	-	1245	WP_000189541.1	esterase FrsA	-
MLA23_RS17770 (3674045)	3674045..3674503	-	459	WP_061349133.1	xanthine phosphoribosyltransferase	-
MLA23_RS17775 (3674764)	3674764..3676221	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
MLA23_RS17780 (3676278)	3676278..3676799	-	522	Protein_3482	peptide chain release factor H	-
MLA23_RS17785 (3676798)	3676798..3677001	-	204	Protein_3483	RtcB family protein	-
MLA23_RS17790 (3677247)	3677247..3677699	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
MLA23_RS17795 (3677709)	3677709..3678107	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
MLA23_RS17800 (3678110)	3678110..3678403	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
MLA23_RS17805 (3678455)	3678455..3679510	-	1056	WP_001226164.1	DNA polymerase IV	-
MLA23_RS17810 (3679581)	3679581..3680366	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
MLA23_RS17815 (3680338)	3680338..3682050	+	1713	Protein_3489	flagellar biosynthesis protein FlhA	-
MLA23_RS17820 (3682274)	3682274..3682771	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T238085 WP_001263493.1 NZ_CP093317:c3678107-3677709 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T238085 NZ_LR890202:4772139-4772549 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT238085 WP_000554757.1 NZ_CP093317:c3678403-3678110 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT238085 NZ_LR890202:4771892-4772158 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6