Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 611799..612598 Replicon chromosome
Accession NZ_CP093317
Organism Escherichia coli strain HUM3355

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag MLA23_RS02960 Protein ID WP_000347251.1
Coordinates 611799..612263 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID D6JF08
Locus tag MLA23_RS02965 Protein ID WP_001308975.1
Coordinates 612263..612598 (-) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MLA23_RS02930 (606800) 606800..607234 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MLA23_RS02935 (607252) 607252..608130 - 879 WP_001300474.1 PTS N-acetylgalactosamine transporter subunit IID -
MLA23_RS02940 (608120) 608120..608899 - 780 WP_001460078.1 PTS N-acetylgalactosamine transporter subunit IIC -
MLA23_RS02945 (608910) 608910..609383 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MLA23_RS02950 (609406) 609406..610686 - 1281 WP_263852504.1 tagatose-bisphosphate aldolase subunit KbaZ -
MLA23_RS02955 (610935) 610935..611744 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MLA23_RS02960 (611799) 611799..612263 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MLA23_RS02965 (612263) 612263..612598 - 336 WP_001308975.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MLA23_RS02970 (612747) 612747..614111 - 1365 Protein_582 galactarate dehydratase -
MLA23_RS02975 (614167) 614167..614864 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
MLA23_RS02980 (614880) 614880..615095 - 216 Protein_584 SAF domain-containing protein -
MLA23_RS02985 (615470) 615470..616804 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MLA23_RS02990 (616820) 616820..617590 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 614487..614864 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T238068 WP_000347251.1 NZ_CP093317:c612263-611799 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T238068 NZ_LR890199:c94401-94096 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12343.97 Da        Isoelectric Point: 4.8616

>AT238068 WP_001308975.1 NZ_CP093317:c612598-612263 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT238068 NZ_LR890199:c94621-94403 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829CJ20


Antitoxin

Source ID Structure
AlphaFold DB D6JF08

References