Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 2403894..2404583 Replicon chromosome
Accession NZ_CP093314
Organism Klebsiella pneumoniae strain HUM7199

Toxin (Protein)


Gene name higB Uniprot ID A0A331C6E2
Locus tag MLA24_RS11775 Protein ID WP_021469727.1
Coordinates 2403894..2404211 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6B0N7G3
Locus tag MLA24_RS11780 Protein ID WP_020804705.1
Coordinates 2404287..2404583 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MLA24_RS11745 (2399596) 2399596..2400105 + 510 WP_020802144.1 GNAT family N-acetyltransferase -
MLA24_RS11750 (2400115) 2400115..2401041 + 927 WP_032435210.1 amino acid ABC transporter permease -
MLA24_RS11755 (2401025) 2401025..2401804 + 780 WP_002906697.1 amino acid ABC transporter ATP-binding protein -
MLA24_RS11760 (2401843) 2401843..2402694 + 852 WP_072198177.1 transporter substrate-binding domain-containing protein -
MLA24_RS11765 (2402772) 2402772..2403389 + 618 WP_032425015.1 glutathione S-transferase family protein -
MLA24_RS11770 (2403460) 2403460..2403687 + 228 WP_002906690.1 tautomerase PptA -
MLA24_RS11775 (2403894) 2403894..2404211 + 318 WP_021469727.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MLA24_RS11780 (2404287) 2404287..2404583 + 297 WP_020804705.1 NadS family protein Antitoxin
MLA24_RS11785 (2404662) 2404662..2405108 + 447 WP_032435212.1 hypothetical protein -
MLA24_RS11790 (2405149) 2405149..2406765 - 1617 WP_004175961.1 carbohydrate porin -
MLA24_RS11795 (2406809) 2406809..2408191 - 1383 WP_004151222.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12221.10 Da        Isoelectric Point: 10.2217

>T238059 WP_021469727.1 NZ_CP093314:2403894-2404211 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Download         Length: 318 bp

>T238059 NZ_LR890198:4186736-4186888 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11057.93 Da        Isoelectric Point: 6.7277

>AT238059 WP_020804705.1 NZ_CP093314:2404287-2404583 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMLMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRHPTGAARK
LLLLLQGNPLIINLLKVV

Download         Length: 297 bp

>AT238059 NZ_LR890198:c4186687-4186630 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331C6E2

Antitoxin

Source ID Structure
AlphaFold DB A0A6B0N7G3

References