Detailed information of TA system TA023805

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 2117770..2119110
• Replicon: chromosome
• Accession: NC_012691
• Organism: Tolumonas auensis DSM 9187

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: C4LG18
• Locus tag: TOLA_RS09850
• Protein ID: WP_015879003.1
• Coordinates: 2118082..2119110 (+)
• Length: 343 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: C4LG17
• Locus tag: TOLA_RS09845
• Protein ID: WP_015879002.1
• Coordinates: 2117770..2118081 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOLA_RS09820	2112968..2114116	-	1149	WP_015878997.1	iron-containing alcohol dehydrogenase	-
TOLA_RS09825	2114262..2114681	-	420	WP_148210498.1	hypothetical protein	-
TOLA_RS09830	2114773..2115879	-	1107	WP_015878999.1	alpha/beta hydrolase	-
TOLA_RS09835	2116174..2117124	+	951	WP_015879000.1	AraC family transcriptional regulator	-
TOLA_RS16535	2117603..2117785	+	183	WP_083757795.1	helix-turn-helix transcriptional regulator	-
TOLA_RS09845	2117770..2118081	+	312	WP_015879002.1	HipA N-terminal domain-containing protein	Antitoxin
TOLA_RS09850	2118082..2119110	+	1029	WP_015879003.1	HipA domain-containing protein	Toxin
TOLA_RS09855	2119332..2119622	+	291	WP_015879004.1	hypothetical protein	-
TOLA_RS09860	2119821..2120129	+	309	WP_015879005.1	copper-binding protein	-
TOLA_RS09865	2120229..2121089	-	861	WP_015879006.1	ribokinase	-
TOLA_RS09870	2121091..2121948	-	858	WP_015879007.1	MurR/RpiR family transcriptional regulator	-
TOLA_RS09875	2121948..2122919	-	972	WP_015879008.1	ribose ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2101371..2131453	30082

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-304)

Antitoxin

• HipA, N-terminal subdomain 1 (6-98)

Sequences

Toxin

Protein

• Length: 343 a.a.
• Molecular weight: 39094.95 Da
• Isoelectric Point: 5.9854

>T23805 WP_015879003.1 NC_012691:2118082-2119110 [Tolumonas auensis DSM 9187]
MDRCLITLELLDKKQPQPGYSSRGIKYLTGNITTSMELPFTRDEFITELPGLQQGLSISGYQPKLSLAIQNKQFHVVDQM
GSYILKPSPEAYPYLAENEHATMMVMKKLKFSVPEFGLVAFKKVDNADPAEFAFIIKRFDRDMKTGEKIHQEQLDGAMAI
DDKYGHRDGKQQISYESACRFLIEQVDSSLPFKRDLFLRVIYAYLLGNNDFHLRNFGILEPQVAPNLLAPVYDFVSTAPY
SSTFNSCYLALPLLTREENDRALAPGFDTQFGQYIGYDFIAFGKGIGLNERYILKLFAEIQKQADVVRDTYQISFMPPQH
IEAVLKCFNTRLKLIEVVDLDR

Nucleotide

• Length: 1029 bp

>T23805 NC_012691:2118082-2119110 [Tolumonas auensis DSM 9187]
ATGGACCGTTGTCTGATCACACTAGAGCTACTGGATAAAAAACAGCCGCAGCCGGGCTATTCTTCCCGTGGGATCAAGTA
TCTGACCGGAAACATAACGACATCCATGGAGCTCCCTTTTACTCGCGACGAGTTCATTACTGAATTACCTGGCTTACAAC
AAGGACTCAGTATTTCCGGTTATCAGCCTAAATTGTCATTAGCCATTCAGAATAAGCAATTTCATGTGGTTGATCAGATG
GGAAGCTACATACTAAAACCATCACCAGAAGCTTATCCCTATTTAGCTGAAAATGAACATGCCACGATGATGGTCATGAA
AAAGCTTAAATTTTCAGTCCCTGAGTTTGGTTTGGTCGCGTTTAAAAAAGTAGATAACGCAGATCCTGCCGAGTTTGCAT
TCATCATTAAACGATTTGATCGTGATATGAAAACTGGTGAAAAAATTCATCAAGAGCAATTGGATGGTGCCATGGCCATT
GATGATAAATACGGTCATCGCGACGGAAAACAACAGATCAGCTATGAATCAGCTTGTCGTTTTCTGATTGAGCAAGTCGA
CAGTAGTTTACCTTTCAAGCGAGATCTGTTTTTACGTGTGATTTATGCTTATTTACTGGGTAACAATGACTTTCATCTGC
GCAATTTTGGTATTCTCGAACCACAAGTCGCACCGAATTTACTGGCACCTGTTTATGATTTTGTCAGTACGGCGCCCTAC
TCTTCTACGTTTAATAGCTGTTATTTGGCGTTGCCATTGCTAACTCGTGAAGAGAATGACAGAGCACTTGCTCCAGGATT
TGATACGCAGTTCGGTCAGTATATTGGTTACGACTTTATAGCGTTCGGAAAAGGGATCGGCTTGAATGAACGGTATATTC
TGAAGTTATTTGCTGAAATTCAAAAGCAAGCGGATGTAGTGCGAGATACTTACCAAATCAGTTTTATGCCGCCACAGCAT
ATTGAAGCTGTACTAAAGTGCTTCAATACAAGATTAAAGCTGATAGAGGTTGTCGATCTCGACCGGTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11835.56 Da
• Isoelectric Point: 7.1630

>AT23805 WP_015879002.1 NC_012691:2117770-2118081 [Tolumonas auensis DSM 9187]
MGRKVIVFCYGKQAGILSENNDEYVFTYDDEYRGKPISLSLPLSKKTFPSKGLHPFFKTLAPEGWLKKRYSELQHIDERD
LFGFLIRNGSDLLGAITFLEVND

Nucleotide

• Length: 312 bp

>AT23805 NC_012691:2117770-2118081 [Tolumonas auensis DSM 9187]
ATGGGCCGCAAAGTAATTGTGTTTTGCTATGGCAAGCAGGCCGGTATTCTTAGTGAAAACAACGACGAATACGTATTCAC
CTATGATGATGAATATAGAGGCAAACCTATCTCTCTCAGCTTGCCATTATCGAAAAAAACATTCCCCAGCAAAGGATTAC
ATCCATTTTTCAAAACCCTGGCACCTGAGGGGTGGTTGAAAAAAAGGTACTCCGAACTCCAACATATTGATGAGCGTGAT
TTATTTGGCTTTTTGATCCGAAACGGCAGTGACTTGTTGGGCGCGATTACTTTTCTTGAGGTTAATGATTAA

Structures

Toxin

• AlphaFold DB: C4LG18

Antitoxin

• AlphaFold DB: C4LG17