Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 350614..351260 Replicon chromosome
Accession NZ_CP093314
Organism Klebsiella pneumoniae strain HUM7199

Toxin (Protein)


Gene name higB Uniprot ID W9BBY1
Locus tag MLA24_RS01620 Protein ID WP_016529833.1
Coordinates 350614..350961 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag MLA24_RS01625 Protein ID WP_002920557.1
Coordinates 350961..351260 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MLA24_RS01610 (346550) 346550..347983 + 1434 WP_002920564.1 glycogen synthase GlgA -
MLA24_RS01615 (348001) 348001..350448 + 2448 WP_002920561.1 glycogen phosphorylase -
MLA24_RS01620 (350614) 350614..350961 + 348 WP_016529833.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MLA24_RS01625 (350961) 350961..351260 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
MLA24_RS01630 (351323) 351323..352831 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
MLA24_RS01635 (352979) 352979..353143 - 165 WP_263852708.1 hypothetical protein -
MLA24_RS01640 (353081) 353081..353363 + 283 Protein_323 thiosulfate sulfurtransferase GlpE -
MLA24_RS01645 (353414) 353414..354244 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
MLA24_RS01650 (354294) 354294..355052 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13549.56 Da        Isoelectric Point: 5.6749

>T238049 WP_016529833.1 NZ_CP093314:350614-350961 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T238049 NZ_LR890198:1981863-1982117 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT238049 WP_002920557.1 NZ_CP093314:350961-351260 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT238049 NZ_LR890198:1981615-1981866 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BBY1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References