Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 324435..325021 Replicon chromosome
Accession NZ_CP093314
Organism Klebsiella pneumoniae strain HUM7199

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag MLA24_RS01510 Protein ID WP_002920800.1
Coordinates 324653..325021 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag MLA24_RS01505 Protein ID WP_004174006.1
Coordinates 324435..324656 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MLA24_RS01485 (320592) 320592..321518 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
MLA24_RS01490 (321515) 321515..322792 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
MLA24_RS01495 (322789) 322789..323556 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
MLA24_RS01500 (323558) 323558..324271 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
MLA24_RS01505 (324435) 324435..324656 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MLA24_RS01510 (324653) 324653..325021 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MLA24_RS01515 (325294) 325294..326610 + 1317 WP_263852664.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
MLA24_RS01520 (326717) 326717..327604 + 888 WP_263852665.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
MLA24_RS01525 (327601) 327601..328446 + 846 WP_032434559.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
MLA24_RS01530 (328448) 328448..329518 + 1071 WP_263852666.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 321515..330255 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T238048 WP_002920800.1 NZ_CP093314:324653-325021 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T238048 NZ_LR890198:1981615-1981866 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT238048 WP_004174006.1 NZ_CP093314:324435-324656 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT238048 NZ_LR890198:1981863-1982117 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGATCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References