Detailed information of TA system TA023804

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 1986978..1987611
• Replicon: chromosome
• Accession: NC_012691
• Organism: Tolumonas auensis DSM 9187

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C4LFQ9
• Locus tag: TOLA_RS09230
• Protein ID: WP_015878897.1
• Coordinates: 1986978..1987310 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: TOLA_RS09235
• Protein ID: WP_015878898.1
• Coordinates: 1987297..1987611 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOLA_RS09200	1982931..1983479	+	549	WP_041609821.1	hypothetical protein	-
TOLA_RS09205	1983593..1984174	+	582	WP_041609505.1	hypothetical protein	-
TOLA_RS09210	1984329..1984607	-	279	WP_015878893.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
TOLA_RS09215	1984611..1984889	-	279	WP_015878894.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
TOLA_RS09220	1985048..1985686	+	639	WP_015878895.1	NAD(P)H-dependent oxidoreductase	-
TOLA_RS09225	1986118..1986426	-	309	WP_015878896.1	copper-binding protein	-
TOLA_RS16680	1986825..1986971	+	147	WP_148210440.1	PAAR domain-containing protein	-
TOLA_RS09230	1986978..1987310	+	333	WP_015878897.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TOLA_RS09235	1987297..1987611	+	315	WP_015878898.1	DNA-binding transcriptional regulator	Antitoxin
TOLA_RS09240	1987734..1988165	+	432	WP_015878899.1	DedA family protein	-
TOLA_RS09245	1988170..1988517	-	348	WP_015878900.1	hypothetical protein	-
TOLA_RS09250	1988685..1989149	+	465	WP_015878901.1	Rrf2 family transcriptional regulator	-
TOLA_RS09255	1989154..1989897	-	744	WP_015878902.1	ferredoxin--NADP(+) reductase	-
TOLA_RS09260	1989978..1991123	-	1146	WP_015878903.1	NADH:flavorubredoxin reductase NorW	-
TOLA_RS09265	1991137..1992552	-	1416	WP_015878904.1	anaerobic nitric oxide reductase flavorubredoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (10-104)

Antitoxin

• Antitoxin MqsA/HigA-2 (18-101)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12854.67 Da
• Isoelectric Point: 9.2240

>T23804 WP_015878897.1 NC_012691:1986978-1987310 [Tolumonas auensis DSM 9187]
MKSIFVESTIFEKYRESYLTDDEFSLFQAELMSDPKKGEVIQGTGGLRKIRVAGKDKGKRGGSRVIYYFLDDLQRFYLLT
IYSKNEMSDLTADQKKQLKAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T23804 NC_012691:1986978-1987310 [Tolumonas auensis DSM 9187]
ATGAAAAGTATCTTTGTTGAATCAACCATTTTTGAAAAGTACCGTGAGTCGTATTTGACTGACGATGAGTTCAGCCTTTT
CCAAGCAGAGCTTATGTCTGATCCCAAGAAAGGCGAGGTGATCCAAGGTACTGGTGGTTTGCGGAAAATTCGAGTCGCAG
GTAAAGATAAGGGCAAACGCGGCGGTTCACGTGTTATCTACTACTTTCTCGATGATTTGCAGCGTTTCTACTTATTGACG
ATTTACAGTAAAAATGAAATGTCTGACTTAACCGCAGATCAAAAGAAACAGTTAAAAGCGTTTATGGAGGCATGGCGCAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11798.38 Da
• Isoelectric Point: 7.0751

>AT23804 WP_015878898.1 NC_012691:1987297-1987611 [Tolumonas auensis DSM 9187]
MSNRDLFAELSSALVEAKEHSEGKLTLKTHQFTNVSELNISPDEIVNIRERFHMSRGVFARLLHTSSRTLENWEQGRSAP
NGQAVTLLKLVQRHPEILAHIAEL

Nucleotide

• Length: 315 bp

>AT23804 NC_012691:1987297-1987611 [Tolumonas auensis DSM 9187]
ATGAGCAATCGTGATTTGTTTGCTGAATTAAGCTCGGCTCTAGTGGAAGCTAAAGAACATTCTGAGGGCAAGCTAACGCT
GAAGACTCATCAGTTCACTAATGTTAGTGAACTCAACATATCTCCTGATGAGATCGTGAATATCCGAGAACGATTTCATA
TGTCTCGCGGCGTATTCGCTCGCCTACTCCATACATCATCAAGAACGCTGGAAAATTGGGAACAAGGCCGTAGTGCACCA
AATGGCCAGGCAGTCACATTACTGAAATTAGTTCAACGCCATCCTGAAATCTTAGCTCACATCGCTGAGCTCTAA

Structures

Toxin

• AlphaFold DB: C4LFQ9