Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3832801..3833342 Replicon chromosome
Accession NZ_CP093283
Organism Escherichia coli strain EC-14-2-9

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag MNY25_RS18575 Protein ID WP_000615983.1
Coordinates 3833064..3833342 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag MNY25_RS18570 Protein ID WP_000729703.1
Coordinates 3832801..3833061 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY25_RS18550 (3828476) 3828476..3829261 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
MNY25_RS18555 (3829233) 3829233..3830945 + 1713 Protein_3655 flagellar biosynthesis protein FlhA -
MNY25_RS18560 (3831169) 3831169..3831666 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
MNY25_RS18565 (3831842) 3831842..3832591 - 750 WP_000056849.1 C40 family peptidase -
MNY25_RS18570 (3832801) 3832801..3833061 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
MNY25_RS18575 (3833064) 3833064..3833342 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
MNY25_RS18580 (3833498) 3833498..3834238 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
MNY25_RS18585 (3834209) 3834209..3834976 - 768 WP_000333380.1 class II glutamine amidotransferase -
MNY25_RS18590 (3835182) 3835182..3835760 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T238014 WP_000615983.1 NZ_CP093283:3833064-3833342 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T238014 NZ_LR890184:3831202-3831420 [Klebsiella aerogenes]
ATGTCTGGTAAACCATTAACTAAAACAGACTATTTGATGCGTTTACGCCGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAGAATAAATATGAACTTTCCGACAATGAATTGGCGGTATTTTACTCAGCGGCCGACCACCGTCTTGCTGAAT
TGACCATGAATAAGCTATACGACAAAATCCCAACTTCAGTATGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT238014 WP_000729703.1 NZ_CP093283:3832801-3833061 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT238014 NZ_LR890184:3830801-3831175 [Klebsiella aerogenes]
ATGGACGAGTACTCGCCAAAAAGACATGATATCGCGCAGCTTAGATTTCTCTGCGAAACCCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCGCGGCTGGGTTAACGATCCCACCTCGGCGGTGAATCTTCAATTAAATGAATTGATCGAGC
ACATAGCAACTTACGCGCTTAATTATAAAATTAAGTACGCTGAGGATAATAAGCTGATTTCACAGCTTGACGAATACCTG
GATGACACTTTTATGCTGTTCAGTAGCTATGGCATAAACACAAACGATCTCCAAAAATGGCGTAAATCAGGTAATCGGTT
ATTCCGATGCTTTGTGAACGCCAGCCGGGAGAACCCGGTAAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References