Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3826604..3827298 Replicon chromosome
Accession NZ_CP093283
Organism Escherichia coli strain EC-14-2-9

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag MNY25_RS18535 Protein ID WP_001263489.1
Coordinates 3826604..3827002 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag MNY25_RS18540 Protein ID WP_000554758.1
Coordinates 3827005..3827298 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3822192) 3822192..3822272 - 81 NuclAT_11 - -
- (3822192) 3822192..3822272 - 81 NuclAT_11 - -
- (3822192) 3822192..3822272 - 81 NuclAT_11 - -
- (3822192) 3822192..3822272 - 81 NuclAT_11 - -
MNY25_RS18510 (3822868) 3822868..3823326 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
MNY25_RS18515 (3823587) 3823587..3825044 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
MNY25_RS18520 (3825101) 3825101..3825622 - 522 Protein_3648 peptide chain release factor H -
MNY25_RS18525 (3825618) 3825618..3825824 - 207 Protein_3649 RtcB family protein -
MNY25_RS18530 (3826142) 3826142..3826594 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
MNY25_RS18535 (3826604) 3826604..3827002 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
MNY25_RS18540 (3827005) 3827005..3827298 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
MNY25_RS18545 (3827350) 3827350..3828405 - 1056 WP_001226164.1 DNA polymerase IV -
MNY25_RS18550 (3828476) 3828476..3829261 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
MNY25_RS18555 (3829233) 3829233..3830945 + 1713 Protein_3655 flagellar biosynthesis protein FlhA -
MNY25_RS18560 (3831169) 3831169..3831666 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T238013 WP_001263489.1 NZ_CP093283:c3827002-3826604 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T238013 NZ_LR890184:2578873-2579358 [Klebsiella aerogenes]
ATGGGAAAAATAAGTGCGCCTGCACCGCTATCTTCACACCATCAGATTGCGGAGTTTTGTTGTGGAGAAACGGTTCTCGA
TCAATGGCTAAAACAACGAGGAATGAAAAACCAGGCTCAGGGAGCCGCGAGAACCTTTGTGGTCTGCAAGGAAGATAGCC
ATCAGGTGGTGGGATTTTATTCGTTAGCAACCGGCAGTGTTAATCACACTGAAGCCACTGGCGGCCTGCGGCGCAATATG
CCCGATCCTATCCCGGTCATCATTCTCGCCCGCCTGGCGATCGACTGCGCGTTTCACGGACAGGGGCTGGGAGCTGATTT
ACTCCATGATGCGCTGTTACGCAGTTATCGCGTTGCCGAAAATGTTGGCGTACGTGCCCTGATGGTCCATGCCCTGACCG
ATAGCGCGAAACGATTCTATTTACACCACGGATTCAAAGCCTCAACCACCCAGGAACGCACACTTTTCCTCGCTTTGCCT
AAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT238013 WP_000554758.1 NZ_CP093283:c3827298-3827005 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT238013 NZ_LR890184:2578619-2578885 [Klebsiella aerogenes]
ATGAAATCAGATATCCAACTCAACATCCGGGCGAAGGAGTCCCAGCGAGCGCTGATTGATGCGGCTGCCGAAATTCTGCA
CAAATCGCGGACCGACTTTATCCTTGAGATGGTCTGCCAGGCGGCAGAAAACGTCATTCTCGACCGTCGGGTGTTTAACT
TAAGCGATGAGCAATACGCCGAATTTATCGACATGCTGGATGCGCCGGTTGAAGCTAACCCCGCTCTCGATAAATTGCTG
GCGAGAAAACCGCAATGGGAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References