Detailed information of TA system TA237995

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 722287..722980
• Replicon: chromosome
• Accession: NZ_CP093283
• Organism: Escherichia coli strain EC-14-2-9

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: MNY25_RS03535
• Protein ID: WP_000415584.1
• Coordinates: 722287..722583 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: MNY25_RS03540
• Protein ID: WP_000650107.1
• Coordinates: 722585..722980 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNY25_RS03500 (717375)	717375..717689	-	315	WP_000958598.1	putative quinol monooxygenase	-
MNY25_RS03505 (717720)	717720..718301	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
MNY25_RS03510 (718620)	718620..718952	+	333	WP_000917685.1	DUF2645 family protein	-
MNY25_RS03515 (718998)	718998..720347	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
MNY25_RS03520 (720344)	720344..721003	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
MNY25_RS03525 (721155)	721155..721547	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
MNY25_RS03530 (721600)	721600..722082	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
MNY25_RS03535 (722287)	722287..722583	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
MNY25_RS03540 (722585)	722585..722980	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
MNY25_RS03545 (723113)	723113..724720	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
MNY25_RS03550 (724858)	724858..727116	+	2259	WP_032329482.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR	rfaE / gspC / gspD / gspE / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / papI	687767..893129	205362

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T237995 WP_000415584.1 NZ_CP093283:722287-722583 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T237995 NZ_LR883965:3980647-3980982 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT237995 WP_000650107.1 NZ_CP093283:722585-722980 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT237995 NZ_LR883965:3980399-3980647 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65