Detailed information of TA system TA237956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 44582..45232
• Replicon: chromosome
• Accession: NZ_CP093271
• Organism: Klebsiella oxytoca strain C-21-18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MN553_RS00210
• Protein ID: WP_049128706.1
• Coordinates: 44582..44923 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MN553_RS00215
• Protein ID: WP_017145203.1
• Coordinates: 44933..45232 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MN553_RS00195 (MN553_00195)	40844..42163	+	1320	WP_004107101.1	MFS transporter	-
MN553_RS00200 (MN553_00200)	42309..43700	+	1392	WP_004107098.1	hexose-6-phosphate:phosphate antiporter	-
MN553_RS00205 (MN553_00205)	43878..44330	+	453	WP_004116616.1	DUF1198 domain-containing protein	-
MN553_RS00210 (MN553_00210)	44582..44923	+	342	WP_049128706.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MN553_RS00215 (MN553_00215)	44933..45232	+	300	WP_017145203.1	helix-turn-helix transcriptional regulator	Antitoxin
MN553_RS00220 (MN553_00220)	45351..46532	+	1182	WP_017145204.1	purine ribonucleoside efflux pump NepI	-
MN553_RS00225 (MN553_00225)	46564..47373	-	810	WP_032729158.1	phosphonoacetaldehyde hydrolase	-
MN553_RS00230 (MN553_00230)	47383..48486	-	1104	WP_049593719.1	2-aminoethylphosphonate--pyruvate transaminase	-
MN553_RS00235 (MN553_00235)	48627..49346	+	720	WP_004107085.1	phosphonate utilization transcriptional regulator PhnR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (8-88)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13064.90 Da
• Isoelectric Point: 5.7456

>T237956 WP_049128706.1 NZ_CP093271:44582-44923 [Klebsiella oxytoca]
MWNVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPQLGRPFADTVNDSAFSNMKELRVQHQGSPIRAFFVFDPSRRGI
LLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Nucleotide

• Length: 342 bp

>T237956 NZ_LR883050:c660712-660248 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10874.80 Da
• Isoelectric Point: 10.0948

>AT237956 WP_017145203.1 NZ_CP093271:44933-45232 [Klebsiella oxytoca]
MATLKELMAKQSPESQARIAEKVEAMRQVIALSMLREELNLSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPGGKRIAFHL

Nucleotide

• Length: 300 bp

>AT237956 NZ_LR883050:c661047-660712 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA