Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 292889..293613 Replicon plasmid pW1-a
Accession NZ_CP093256
Organism Moellerella wisconsensis strain W1

Toxin (Protein)


Gene name higB Uniprot ID A0A4D7JCU9
Locus tag MNY70_RS17825 Protein ID WP_067423691.1
Coordinates 293302..293613 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4D7J260
Locus tag MNY70_RS17820 Protein ID WP_067423690.1
Coordinates 292889..293305 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY70_RS17780 (MNY70_17780) 288018..288440 + 423 WP_137022573.1 hypothetical protein -
MNY70_RS17785 (MNY70_17785) 288609..289166 + 558 WP_137022574.1 hypothetical protein -
MNY70_RS17790 (MNY70_17790) 289305..289874 + 570 WP_241502031.1 GDSL-type esterase/lipase family protein -
MNY70_RS17795 (MNY70_17795) 290211..290708 + 498 WP_137022577.1 hypothetical protein -
MNY70_RS17800 (MNY70_17800) 290809..291192 + 384 WP_137022578.1 hypothetical protein -
MNY70_RS17805 (MNY70_17805) 291256..292032 + 777 WP_067421877.1 methyltransferase -
MNY70_RS17810 (MNY70_17810) 292164..292499 + 336 WP_137022579.1 hypothetical protein -
MNY70_RS17815 (MNY70_17815) 292525..292830 + 306 WP_137022580.1 hypothetical protein -
MNY70_RS17820 (MNY70_17820) 292889..293305 - 417 WP_067423690.1 helix-turn-helix domain-containing protein Antitoxin
MNY70_RS17825 (MNY70_17825) 293302..293613 - 312 WP_067423691.1 type II toxin-antitoxin system HigB family toxin Toxin
MNY70_RS17830 (MNY70_17830) 293784..294350 + 567 WP_067423693.1 hypothetical protein -
MNY70_RS17835 (MNY70_17835) 294473..294790 + 318 WP_067423694.1 hypothetical protein -
MNY70_RS17840 (MNY70_17840) 294787..295092 + 306 WP_067423696.1 hypothetical protein -
MNY70_RS17845 (MNY70_17845) 295398..296270 + 873 WP_235845366.1 repA protein -
MNY70_RS17850 (MNY70_17850) 296811..297296 + 486 WP_067423698.1 hypothetical protein -
MNY70_RS17855 (MNY70_17855) 297360..297809 + 450 WP_137022583.1 hypothetical protein -
MNY70_RS17860 (MNY70_17860) 297980..298462 + 483 WP_137022584.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(A) / blaCTX-M-1 / floR / aph(6)-Id / aph(3'')-Ib / aadA14 / lnu(G) / sul2 - 1..331804 331804


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(82-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12375.16 Da        Isoelectric Point: 10.3654

>T237950 WP_067423691.1 NZ_CP093256:c293613-293302 [Moellerella wisconsensis]
MHVISRDPFNQASKRFPNSAQALSDVYRTLKRDSYQTPDELKKVFPSLDRMKYREKWWVIDISGNALRMMFFADFDRGKI
FVKHITTHAEYDKLTDHYRRTKA

Download         Length: 312 bp

>T237950 NZ_LR883012:c4151134-4150847 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15451.44 Da        Isoelectric Point: 4.6155

>AT237950 WP_067423690.1 NZ_CP093256:c293305-292889 [Moellerella wisconsensis]
MMFQDAVKAAQNLVNLVPLLGDSHSRPDYEKAVQLVEHLVENDPDNPLIDMICAKIDAYENAAPEFAEFNQRLAESNDGV
AALRTLMDQYNLNTTDFGNELGSRSYVSRILNGERGLTLEHIKNLSKRFGIPASIFIR

Download         Length: 417 bp

>AT237950 NZ_LR883012:c4150860-4150558 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D7JCU9

Antitoxin

Source ID Structure
AlphaFold DB A0A4D7J260

References