Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 116740..117391 Replicon plasmid pW18-2-a
Accession NZ_CP093250
Organism Moellerella wisconsensis strain W18-2

Toxin (Protein)


Gene name higB Uniprot ID A0A1B8HBJ1
Locus tag MNY64_RS16730 Protein ID WP_067423956.1
Coordinates 117041..117391 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1B8HBM2
Locus tag MNY64_RS16725 Protein ID WP_067423953.1
Coordinates 116740..117039 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY64_RS16670 (MNY64_16680) 112040..112340 - 301 Protein_140 transposase -
MNY64_RS16675 (MNY64_16685) 112377..112577 - 201 Protein_141 PAAR domain-containing protein -
MNY64_RS16680 (MNY64_16690) 112592..113140 - 549 WP_082981383.1 PAAR domain-containing protein -
MNY64_RS16685 (MNY64_16695) 113357..113566 + 210 WP_109910748.1 hypothetical protein -
MNY64_RS16690 (MNY64_16700) 113643..113957 + 315 WP_082981384.1 hypothetical protein -
MNY64_RS16695 113938..114174 + 237 WP_067423943.1 hypothetical protein -
MNY64_RS16700 (MNY64_16705) 114231..114449 + 219 WP_109910747.1 hypothetical protein -
MNY64_RS16705 (MNY64_16710) 114483..115007 + 525 WP_067423945.1 hypothetical protein -
MNY64_RS16710 114961..115338 - 378 WP_109910745.1 hypothetical protein -
MNY64_RS16715 (MNY64_16720) 115439..115840 + 402 WP_067423949.1 hypothetical protein -
MNY64_RS16720 (MNY64_16725) 115875..116711 - 837 WP_109910744.1 hypothetical protein -
MNY64_RS16725 (MNY64_16730) 116740..117039 - 300 WP_067423953.1 helix-turn-helix domain-containing protein Antitoxin
MNY64_RS16730 (MNY64_16735) 117041..117391 - 351 WP_067423956.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MNY64_RS16735 (MNY64_16740) 117587..118147 + 561 WP_213914584.1 recombinase family protein -
MNY64_RS16740 (MNY64_16745) 118689..119591 + 903 WP_241502041.1 WYL domain-containing protein -
MNY64_RS16745 (MNY64_16750) 119601..120200 + 600 WP_241543888.1 DUF1819 family protein -
MNY64_RS16750 (MNY64_16755) 120197..120796 + 600 WP_241542955.1 DUF1788 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(3'')-Ia / lnu(F) / floR / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-1 / mph(A) - 1..270793 270793
- flank IS/Tn - - 117587..118147 560


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-84)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13453.50 Da        Isoelectric Point: 5.2661

>T237942 WP_067423956.1 NZ_CP093250:c117391-117041 [Moellerella wisconsensis]
MWEILTRDLFDSWFEEQNEETQIEVLAVLMILREDGPNLGRPQVDTLKGSQFPNMKELRIQVGGHPIRACFAFDPIRRGI
VLCAGDKKGKDETRFYKKLIKMADAEYSAHLLEQEK

Download         Length: 351 bp

>T237942 NZ_LR883012:2099537-2099640 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10888.61 Da        Isoelectric Point: 8.5841

>AT237942 WP_067423953.1 NZ_CP093250:c117039-116740 [Moellerella wisconsensis]
MSTLNELLAKQSPEALAKIEARAEEIRREITLAKIREELNLSQSELAKSLGVSQPSIAKLENVDNDPKLSTLKRYIKALG
GELSIDVTLPNGKRIGLHL

Download         Length: 300 bp

>AT237942 NZ_LR883012:c2099678-2099407 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B8HBJ1

Antitoxin

Source ID Structure
AlphaFold DB A0A1B8HBM2

References