Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 53169..53893 Replicon plasmid pW18-2-a
Accession NZ_CP093250
Organism Moellerella wisconsensis strain W18-2

Toxin (Protein)


Gene name higB Uniprot ID A0A4D7JCU9
Locus tag MNY64_RS16320 Protein ID WP_067423691.1
Coordinates 53582..53893 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4D7J260
Locus tag MNY64_RS16315 Protein ID WP_067423690.1
Coordinates 53169..53585 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY64_RS16275 (MNY64_16285) 48298..48720 + 423 WP_137022573.1 hypothetical protein -
MNY64_RS16280 (MNY64_16290) 48889..49446 + 558 WP_137022574.1 hypothetical protein -
MNY64_RS16285 (MNY64_16295) 49585..50154 + 570 WP_241502031.1 GDSL-type esterase/lipase family protein -
MNY64_RS16290 (MNY64_16300) 50491..50988 + 498 WP_137022577.1 hypothetical protein -
MNY64_RS16295 (MNY64_16305) 51047..51472 + 426 WP_067421875.1 hypothetical protein -
MNY64_RS16300 (MNY64_16310) 51536..52312 + 777 WP_067421877.1 methyltransferase -
MNY64_RS16305 (MNY64_16315) 52444..52779 + 336 WP_137022579.1 hypothetical protein -
MNY64_RS16310 (MNY64_16320) 52805..53110 + 306 WP_137022580.1 hypothetical protein -
MNY64_RS16315 (MNY64_16325) 53169..53585 - 417 WP_067423690.1 helix-turn-helix domain-containing protein Antitoxin
MNY64_RS16320 (MNY64_16330) 53582..53893 - 312 WP_067423691.1 type II toxin-antitoxin system HigB family toxin Toxin
MNY64_RS16325 (MNY64_16335) 54064..54630 + 567 WP_067423693.1 hypothetical protein -
MNY64_RS16330 (MNY64_16340) 54753..55070 + 318 WP_067423694.1 hypothetical protein -
MNY64_RS16335 (MNY64_16345) 55067..55372 + 306 WP_067423696.1 hypothetical protein -
MNY64_RS16340 (MNY64_16350) 55678..56550 + 873 WP_235845366.1 repA protein -
MNY64_RS16345 (MNY64_16355) 57091..57576 + 486 WP_067423698.1 hypothetical protein -
MNY64_RS16350 (MNY64_16360) 57640..58089 + 450 WP_137022583.1 hypothetical protein -
MNY64_RS16355 (MNY64_16365) 58260..58742 + 483 WP_137022584.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(3'')-Ia / lnu(F) / floR / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-1 / mph(A) - 1..270793 270793


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(82-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12375.16 Da        Isoelectric Point: 10.3654

>T237941 WP_067423691.1 NZ_CP093250:c53893-53582 [Moellerella wisconsensis]
MHVISRDPFNQASKRFPNSAQALSDVYRTLKRDSYQTPDELKKVFPSLDRMKYREKWWVIDISGNALRMMFFADFDRGKI
FVKHITTHAEYDKLTDHYRRTKA

Download         Length: 312 bp

>T237941 NZ_LR883012:1902937-1903191 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15451.44 Da        Isoelectric Point: 4.6155

>AT237941 WP_067423690.1 NZ_CP093250:c53585-53169 [Moellerella wisconsensis]
MMFQDAVKAAQNLVNLVPLLGDSHSRPDYEKAVQLVEHLVENDPDNPLIDMICAKIDAYENAAPEFAEFNQRLAESNDGV
AALRTLMDQYNLNTTDFGNELGSRSYVSRILNGERGLTLEHIKNLSKRFGIPASIFIR

Download         Length: 417 bp

>AT237941 NZ_LR883012:1902689-1902940 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D7JCU9

Antitoxin

Source ID Structure
AlphaFold DB A0A4D7J260

References