Detailed information of TA system TA237934

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 99593..100317
• Replicon: plasmid pW51-a
• Accession: NZ_CP093246
• Organism: Moellerella wisconsensis strain W51

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4D7JCU9
• Locus tag: MNY72_RS16460
• Protein ID: WP_067423691.1
• Coordinates: 99593..99904 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A4D7J260
• Locus tag: MNY72_RS16465
• Protein ID: WP_067423690.1
• Coordinates: 99901..100317 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNY72_RS16425 (MNY72_16420)	94744..95226	-	483	WP_137022584.1	hypothetical protein	-
MNY72_RS16430 (MNY72_16425)	95397..95846	-	450	WP_137022583.1	hypothetical protein	-
MNY72_RS16435 (MNY72_16430)	95910..96395	-	486	WP_067423698.1	hypothetical protein	-
MNY72_RS16440 (MNY72_16435)	96936..97808	-	873	WP_235845366.1	repA protein	-
MNY72_RS16445 (MNY72_16440)	98114..98419	-	306	WP_067423696.1	hypothetical protein	-
MNY72_RS16450 (MNY72_16445)	98416..98733	-	318	WP_067423694.1	hypothetical protein	-
MNY72_RS16455 (MNY72_16450)	98856..99422	-	567	WP_067423693.1	hypothetical protein	-
MNY72_RS16460 (MNY72_16455)	99593..99904	+	312	WP_067423691.1	type II toxin-antitoxin system HigB family toxin	Toxin
MNY72_RS16465 (MNY72_16460)	99901..100317	+	417	WP_067423690.1	helix-turn-helix domain-containing protein	Antitoxin
MNY72_RS16470 (MNY72_16465)	100376..100681	-	306	WP_137022580.1	hypothetical protein	-
MNY72_RS16475 (MNY72_16470)	100707..101042	-	336	WP_137022579.1	hypothetical protein	-
MNY72_RS16480 (MNY72_16475)	101174..101950	-	777	WP_067421877.1	methyltransferase	-
MNY72_RS16485 (MNY72_16480)	102014..102439	-	426	WP_067421875.1	hypothetical protein	-
MNY72_RS16490 (MNY72_16485)	102498..102995	-	498	WP_137022577.1	hypothetical protein	-
MNY72_RS16495 (MNY72_16490)	103332..103901	-	570	WP_241502031.1	GDSL-type esterase/lipase family protein	-
MNY72_RS16500 (MNY72_16495)	104040..104597	-	558	WP_137022574.1	hypothetical protein	-
MNY72_RS16505 (MNY72_16500)	104766..105188	-	423	WP_137022573.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IIa / aph(3')-Ia / mph(A) / blaCTX-M-1 / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA1 / ant(3'')-Ia / tet(D)	-	1..270632	270632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (82-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12375.16 Da
• Isoelectric Point: 10.3654

>T237934 WP_067423691.1 NZ_CP093246:99593-99904 [Moellerella wisconsensis]
MHVISRDPFNQASKRFPNSAQALSDVYRTLKRDSYQTPDELKKVFPSLDRMKYREKWWVIDISGNALRMMFFADFDRGKI
FVKHITTHAEYDKLTDHYRRTKA

Nucleotide

• Length: 312 bp

>T237934 NZ_LR883012:180405-180512 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15451.44 Da
• Isoelectric Point: 4.6155

>AT237934 WP_067423690.1 NZ_CP093246:99901-100317 [Moellerella wisconsensis]
MMFQDAVKAAQNLVNLVPLLGDSHSRPDYEKAVQLVEHLVENDPDNPLIDMICAKIDAYENAAPEFAEFNQRLAESNDGV
AALRTLMDQYNLNTTDFGNELGSRSYVSRILNGERGLTLEHIKNLSKRFGIPASIFIR

Nucleotide

• Length: 417 bp

>AT237934 NZ_LR883012:c180357-180291 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A4D7JCU9

Antitoxin

• AlphaFold DB: A0A4D7J260