Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 22008..22659 Replicon plasmid pW51-a
Accession NZ_CP093246
Organism Moellerella wisconsensis strain W51

Toxin (Protein)


Gene name higB Uniprot ID A0A1B8HBJ1
Locus tag MNY72_RS15970 Protein ID WP_067423956.1
Coordinates 22008..22358 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1B8HBM2
Locus tag MNY72_RS15975 Protein ID WP_067423953.1
Coordinates 22360..22659 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY72_RS15950 (MNY72_15945) 18611..19210 - 600 WP_241542955.1 DUF1788 domain-containing protein -
MNY72_RS15955 (MNY72_15950) 19207..19806 - 600 WP_277629383.1 DUF1819 family protein -
MNY72_RS15960 (MNY72_15955) 19816..20718 - 903 WP_241542965.1 WYL domain-containing protein -
MNY72_RS15965 (MNY72_15960) 21252..21812 - 561 WP_213914584.1 recombinase family protein -
MNY72_RS15970 (MNY72_15965) 22008..22358 + 351 WP_067423956.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MNY72_RS15975 (MNY72_15970) 22360..22659 + 300 WP_067423953.1 helix-turn-helix domain-containing protein Antitoxin
MNY72_RS15980 (MNY72_15975) 22688..23524 + 837 WP_109910744.1 hypothetical protein -
MNY72_RS15985 (MNY72_15980) 23559..23960 - 402 WP_067423949.1 hypothetical protein -
MNY72_RS15990 (MNY72_15985) 24061..24438 + 378 WP_109910745.1 hypothetical protein -
MNY72_RS15995 (MNY72_15990) 24392..24916 - 525 WP_067423945.1 hypothetical protein -
MNY72_RS16000 (MNY72_15995) 24950..25168 - 219 WP_109910747.1 hypothetical protein -
MNY72_RS16005 (MNY72_16000) 25225..25461 - 237 WP_067423943.1 hypothetical protein -
MNY72_RS16010 (MNY72_16005) 25442..25756 - 315 WP_082981384.1 hypothetical protein -
MNY72_RS16015 (MNY72_16010) 25833..26042 - 210 WP_109910748.1 hypothetical protein -
MNY72_RS16020 (MNY72_16015) 26259..26807 + 549 WP_082981383.1 PAAR domain-containing protein -
MNY72_RS16025 (MNY72_16020) 26822..27022 + 201 Protein_27 PAAR domain-containing protein -
MNY72_RS16030 (MNY72_16025) 27059..27359 + 301 Protein_28 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(3)-IIa / aph(3')-Ia / mph(A) / blaCTX-M-1 / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA1 / ant(3'')-Ia / tet(D) - 1..270632 270632
- flank IS/Tn - - 21252..21812 560


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-84)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13453.50 Da        Isoelectric Point: 5.2661

>T237933 WP_067423956.1 NZ_CP093246:22008-22358 [Moellerella wisconsensis]
MWEILTRDLFDSWFEEQNEETQIEVLAVLMILREDGPNLGRPQVDTLKGSQFPNMKELRIQVGGHPIRACFAFDPIRRGI
VLCAGDKKGKDETRFYKKLIKMADAEYSAHLLEQEK

Download         Length: 351 bp

>T237933 NZ_LR883011:7086-7340 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10888.61 Da        Isoelectric Point: 8.5841

>AT237933 WP_067423953.1 NZ_CP093246:22360-22659 [Moellerella wisconsensis]
MSTLNELLAKQSPEALAKIEARAEEIRREITLAKIREELNLSQSELAKSLGVSQPSIAKLENVDNDPKLSTLKRYIKALG
GELSIDVTLPNGKRIGLHL

Download         Length: 300 bp

>AT237933 NZ_LR883011:7337-7579 [Escherichia coli]
ATGACTATCGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B8HBJ1

Antitoxin

Source ID Structure
AlphaFold DB A0A1B8HBM2

References