Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 192960..193611 Replicon plasmid pW65-a
Accession NZ_CP093243
Organism Moellerella wisconsensis strain W65

Toxin (Protein)


Gene name higB Uniprot ID A0A1B8HBJ1
Locus tag MNY66_RS17020 Protein ID WP_067423956.1
Coordinates 193261..193611 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1B8HBM2
Locus tag MNY66_RS17015 Protein ID WP_067423953.1
Coordinates 192960..193259 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY66_RS16965 (MNY66_16965) 188597..188797 - 201 Protein_245 PAAR domain-containing protein -
MNY66_RS16970 (MNY66_16970) 188812..189360 - 549 WP_241501905.1 PAAR domain-containing protein -
MNY66_RS16975 (MNY66_16975) 189577..189786 + 210 WP_109910748.1 hypothetical protein -
MNY66_RS16980 (MNY66_16980) 189863..190177 + 315 WP_082981384.1 hypothetical protein -
MNY66_RS16985 (MNY66_16985) 190158..190394 + 237 WP_067423943.1 hypothetical protein -
MNY66_RS16990 (MNY66_16990) 190451..190669 + 219 WP_109910747.1 hypothetical protein -
MNY66_RS16995 (MNY66_16995) 190703..191227 + 525 WP_067423945.1 hypothetical protein -
MNY66_RS17000 (MNY66_17000) 191181..191558 - 378 WP_109910745.1 hypothetical protein -
MNY66_RS17005 (MNY66_17005) 191659..192060 + 402 WP_067423949.1 hypothetical protein -
MNY66_RS17010 (MNY66_17010) 192095..192931 - 837 WP_109910744.1 hypothetical protein -
MNY66_RS17015 (MNY66_17015) 192960..193259 - 300 WP_067423953.1 helix-turn-helix domain-containing protein Antitoxin
MNY66_RS17020 (MNY66_17020) 193261..193611 - 351 WP_067423956.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MNY66_RS17025 (MNY66_17025) 193807..194367 + 561 WP_213914584.1 recombinase family protein -
MNY66_RS17030 (MNY66_17030) 194909..195811 + 903 WP_241502041.1 WYL domain-containing protein -
MNY66_RS17035 (MNY66_17035) 195821..196420 + 600 WP_241501906.1 DUF1819 family protein -
MNY66_RS17040 (MNY66_17040) 196417..197016 + 600 WP_213914587.1 DUF1788 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(H) / aadA14 / dfrA1 / ant(3'')-Ia / sul3 / blaCTX-M-1 / mph(A) / aph(3')-Ia - 1..345391 345391
- flank IS/Tn - - 193807..194367 560


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(32-84)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13453.50 Da        Isoelectric Point: 5.2661

>T237926 WP_067423956.1 NZ_CP093243:c193611-193261 [Moellerella wisconsensis]
MWEILTRDLFDSWFEEQNEETQIEVLAVLMILREDGPNLGRPQVDTLKGSQFPNMKELRIQVGGHPIRACFAFDPIRRGI
VLCAGDKKGKDETRFYKKLIKMADAEYSAHLLEQEK

Download         Length: 351 bp

>T237926 NZ_LR883006:c4471368-4471195 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10888.61 Da        Isoelectric Point: 8.5841

>AT237926 WP_067423953.1 NZ_CP093243:c193259-192960 [Moellerella wisconsensis]
MSTLNELLAKQSPEALAKIEARAEEIRREITLAKIREELNLSQSELAKSLGVSQPSIAKLENVDNDPKLSTLKRYIKALG
GELSIDVTLPNGKRIGLHL

Download         Length: 300 bp

>AT237926 NZ_LR883006:c4471692-4471396 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B8HBJ1

Antitoxin

Source ID Structure
AlphaFold DB A0A1B8HBM2

References