Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 154804..155528 Replicon plasmid pW65-a
Accession NZ_CP093243
Organism Moellerella wisconsensis strain W65

Toxin (Protein)


Gene name higB Uniprot ID A0A4D7JCU9
Locus tag MNY66_RS16770 Protein ID WP_067423691.1
Coordinates 155217..155528 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4D7J260
Locus tag MNY66_RS16765 Protein ID WP_067423690.1
Coordinates 154804..155220 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY66_RS16725 (MNY66_16725) 149933..150355 + 423 WP_137022573.1 hypothetical protein -
MNY66_RS16730 (MNY66_16730) 150524..151081 + 558 WP_137022574.1 hypothetical protein -
MNY66_RS16735 (MNY66_16735) 151220..151789 + 570 WP_241502031.1 GDSL-type esterase/lipase family protein -
MNY66_RS16740 (MNY66_16740) 152126..152623 + 498 WP_137022577.1 hypothetical protein -
MNY66_RS16745 (MNY66_16745) 152682..153107 + 426 WP_067421875.1 hypothetical protein -
MNY66_RS16750 (MNY66_16750) 153171..153947 + 777 WP_067421877.1 methyltransferase -
MNY66_RS16755 (MNY66_16755) 154079..154414 + 336 WP_137022579.1 hypothetical protein -
MNY66_RS16760 (MNY66_16760) 154440..154745 + 306 WP_137022580.1 hypothetical protein -
MNY66_RS16765 (MNY66_16765) 154804..155220 - 417 WP_067423690.1 helix-turn-helix domain-containing protein Antitoxin
MNY66_RS16770 (MNY66_16770) 155217..155528 - 312 WP_067423691.1 type II toxin-antitoxin system HigB family toxin Toxin
MNY66_RS16775 (MNY66_16775) 155699..156265 + 567 WP_067423693.1 hypothetical protein -
MNY66_RS16780 (MNY66_16780) 156388..156705 + 318 WP_067423694.1 hypothetical protein -
MNY66_RS16785 (MNY66_16785) 156702..157007 + 306 WP_067423696.1 hypothetical protein -
MNY66_RS16790 (MNY66_16790) 157313..158185 + 873 WP_235845366.1 repA protein -
MNY66_RS16795 (MNY66_16795) 158726..159211 + 486 WP_067423698.1 hypothetical protein -
MNY66_RS16800 (MNY66_16800) 159275..159724 + 450 WP_137022583.1 hypothetical protein -
MNY66_RS16805 (MNY66_16805) 159895..160377 + 483 WP_137022584.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(H) / aadA14 / dfrA1 / ant(3'')-Ia / sul3 / blaCTX-M-1 / mph(A) / aph(3')-Ia - 1..345391 345391


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(82-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12375.16 Da        Isoelectric Point: 10.3654

>T237925 WP_067423691.1 NZ_CP093243:c155528-155217 [Moellerella wisconsensis]
MHVISRDPFNQASKRFPNSAQALSDVYRTLKRDSYQTPDELKKVFPSLDRMKYREKWWVIDISGNALRMMFFADFDRGKI
FVKHITTHAEYDKLTDHYRRTKA

Download         Length: 312 bp

>T237925 NZ_LR883006:4271415-4271765 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15451.44 Da        Isoelectric Point: 4.6155

>AT237925 WP_067423690.1 NZ_CP093243:c155220-154804 [Moellerella wisconsensis]
MMFQDAVKAAQNLVNLVPLLGDSHSRPDYEKAVQLVEHLVENDPDNPLIDMICAKIDAYENAAPEFAEFNQRLAESNDGV
AALRTLMDQYNLNTTDFGNELGSRSYVSRILNGERGLTLEHIKNLSKRFGIPASIFIR

Download         Length: 417 bp

>AT237925 NZ_LR883006:4271170-4271421 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D7JCU9

Antitoxin

Source ID Structure
AlphaFold DB A0A4D7J260

References