Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2369581..2370143 Replicon chromosome
Accession NZ_CP093239
Organism Escherichia marmotae strain W49-2

Toxin (Protein)


Gene name higB Uniprot ID A0A7H9K4H3
Locus tag MNY74_RS11180 Protein ID WP_001516386.1
Coordinates 2369581..2369859 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag MNY74_RS11185 Protein ID WP_000781370.1
Coordinates 2369859..2370143 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY74_RS11150 (2365091) 2365091..2365459 - 369 WP_241556839.1 omptin family outer membrane protease -
MNY74_RS11155 (2365420) 2365420..2365632 - 213 Protein_2187 peroxiredoxin OsmC -
MNY74_RS11160 (2365955) 2365955..2366170 + 216 WP_000495761.1 biofilm-dependent modulation protein -
MNY74_RS11165 (2366261) 2366261..2366398 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
MNY74_RS11170 (2366554) 2366554..2368251 + 1698 WP_001516384.1 malate dehydrogenase -
MNY74_RS11175 (2368385) 2368385..2369395 + 1011 WP_000642389.1 alcohol dehydrogenase AdhP -
MNY74_RS11180 (2369581) 2369581..2369859 + 279 WP_001516386.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MNY74_RS11185 (2369859) 2369859..2370143 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
MNY74_RS11190 (2370371) 2370371..2371024 - 654 WP_000045637.1 formate dehydrogenase-N subunit gamma -
MNY74_RS11195 (2371017) 2371017..2371901 - 885 WP_001240589.1 formate dehydrogenase N subunit beta -
MNY74_RS11200 (2371914) 2371914..2374961 - 3048 WP_077633351.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10623.19 Da        Isoelectric Point: 6.8682

>T237908 WP_001516386.1 NZ_CP093239:2369581-2369859 [Escherichia marmotae]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDEIWTISVSGNWRLTFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T237908 NZ_LR883006:1045842-1046177 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT237908 WP_000781370.1 NZ_CP093239:2369859-2370143 [Escherichia marmotae]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT237908 NZ_LR883006:1045594-1045842 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H9K4H3

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References