Detailed information of TA system TA237878

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 57593..57847
• Replicon: plasmid pEC-16-35-2
• Accession: NZ_CP093227
• Organism: Escherichia coli strain EC-16-35

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A1D7QA06
• Locus tag: MNY24_RS23410
• Protein ID: WP_001367749.1
• Coordinates: 57698..57847 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	57593..57654 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNY24_RS23380 (53353)	53353..54099	+	747	WP_052273601.1	conjugal transfer pilus acetylase TraX	-
MNY24_RS23385 (54154)	54154..54714	+	561	WP_001567328.1	fertility inhibition protein FinO	-
MNY24_RS23390 (54845)	54845..55054	+	210	WP_039023209.1	hypothetical protein	-
MNY24_RS23395 (55466)	55466..56092	+	627	WP_029702149.1	NYN domain-containing protein	-
MNY24_RS23400 (56244)	56244..56813	+	570	WP_029702150.1	DUF2726 domain-containing protein	-
MNY24_RS23405 (57026)	57026..57454	+	429	WP_029702151.1	hypothetical protein	-
- (57593)	57593..57654	-	62	NuclAT_2	-	Antitoxin
- (57593)	57593..57654	-	62	NuclAT_2	-	Antitoxin
- (57593)	57593..57654	-	62	NuclAT_2	-	Antitoxin
- (57593)	57593..57654	-	62	NuclAT_2	-	Antitoxin
MNY24_RS23410 (57698)	57698..57847	+	150	WP_001367749.1	Hok/Gef family protein	Toxin
MNY24_RS23415 (58131)	58131..58388	+	258	WP_000083833.1	replication regulatory protein RepA	-
MNY24_RS23420 (58624)	58624..58698	+	75	WP_001365705.1	RepA leader peptide Tap	-
MNY24_RS23425 (58691)	58691..59548	+	858	WP_029702152.1	incFII family plasmid replication initiator RepA	-
MNY24_RS23430 (60249)	60249..60389	+	141	WP_001333237.1	hypothetical protein	-
MNY24_RS23435 (60487)	60487..61140	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
MNY24_RS23440 (61233)	61233..61490	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
MNY24_RS23445 (61423)	61423..61824	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA17 / aadA5 / qacE / sul1 / mph(A) / floR / aph(6)-Id / aph(3'')-Ib / sul2 / erm(B) / blaTEM-1B / rmtB / fosA3 / blaCTX-M-55	-	1..212822	212822

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5528.64 Da
• Isoelectric Point: 8.7678

>T237878 WP_001367749.1 NZ_CP093227:57698-57847 [Escherichia coli]
MTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T237878 NZ_LR882997:1074215-1074550 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Nucleotide

• Length: 62 bp

>AT237878 NZ_CP093227:c57654-57593 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A1D7QA06

Antitoxin