Detailed information of TA system TA237874

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 16127..16548
• Replicon: plasmid pEC-16-35-2
• Accession: NZ_CP093227
• Organism: Escherichia coli strain EC-16-35

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MNY24_RS23175
• Protein ID: WP_096937776.1
• Coordinates: 16423..16548 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	16127..16325 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNY24_RS23135 (11823)	11823..13217	+	1395	WP_001037799.1	carbohydrate porin	-
MNY24_RS23140 (13463)	13463..13567	-	105	Protein_11	thiosulfate reductase cytochrome B subunit	-
MNY24_RS23145 (13624)	13624..14317	+	694	Protein_12	IS1 family transposase	-
MNY24_RS23150 (14444)	14444..14956	+	513	WP_223350513.1	potassium channel family protein	-
MNY24_RS23155 (15041)	15041..15292	+	252	Protein_14	iron-containing alcohol dehydrogenase	-
MNY24_RS23160 (15581)	15581..15898	-	318	Protein_15	IS110 family transposase	-
MNY24_RS23165 (16053)	16053..16158	+	106	Protein_16	plasmid SOS inhibition protein A	-
- (16127)	16127..16325	+	199	NuclAT_1	-	Antitoxin
- (16127)	16127..16325	+	199	NuclAT_1	-	Antitoxin
- (16127)	16127..16325	+	199	NuclAT_1	-	Antitoxin
- (16127)	16127..16325	+	199	NuclAT_1	-	Antitoxin
MNY24_RS23170 (16332)	16332..16481	+	150	Protein_17	DUF5431 family protein	-
MNY24_RS23175 (16423)	16423..16548	+	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
MNY24_RS23180 (16849)	16849..17146	-	298	Protein_19	hypothetical protein	-
MNY24_RS23185 (17216)	17216..17422	+	207	WP_000275854.1	hypothetical protein	-
MNY24_RS23190 (17447)	17447..17734	+	288	WP_000107242.1	hypothetical protein	-
MNY24_RS23195 (17853)	17853..18674	+	822	WP_001234465.1	DUF932 domain-containing protein	-
MNY24_RS23200 (18970)	18970..19542	-	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
MNY24_RS23205 (19912)	19912..20295	+	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MNY24_RS23210 (20486)	20486..21133	+	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
MNY24_RS23215 (21269)	21269..21484	+	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA17 / aadA5 / qacE / sul1 / mph(A) / floR / aph(6)-Id / aph(3'')-Ib / sul2 / erm(B) / blaTEM-1B / rmtB / fosA3 / blaCTX-M-55	-	1..212822	212822
	-	flank	IS/Tn	-	-	13940..14317	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T237874 WP_096937776.1 NZ_CP093227:16423-16548 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T237874 NZ_LR882991:c124524-124198 [Escherichia coli]
ATGACTTACACAGTCAAATTCAGGGATGATGCACTGAAAGAATGGCTGAAACTGGATAAGGCCATTCAACAGCAATTTGC
TAAAAAGCTAAAGAAGTGCAGTGAAAATCCTCATATTCCCTCTGCTAAGCTGCGCGGGATAAAAGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATAGATGATCAATTAATTATCGCTGTTGTTGCTATTGGGAAA
CGTGAACGTAGTGATGTTTATAATCTTGCCAGCCGAGTAGGCAGCCTGGCGGCTGCGGCTTGTCATGGACTGAAATTACC
GTTATAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT237874 NZ_CP093227:16127-16325 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin