Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3715951..3716645 Replicon chromosome
Accession NZ_CP093226
Organism Escherichia coli strain EC-16-35

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag MNY24_RS17925 Protein ID WP_001263489.1
Coordinates 3715951..3716349 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag MNY24_RS17930 Protein ID WP_000554758.1
Coordinates 3716352..3716645 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- (3711539) 3711539..3711619 - 81 NuclAT_11 - -
- (3711539) 3711539..3711619 - 81 NuclAT_11 - -
- (3711539) 3711539..3711619 - 81 NuclAT_11 - -
- (3711539) 3711539..3711619 - 81 NuclAT_11 - -
MNY24_RS17900 (3712215) 3712215..3712673 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
MNY24_RS17905 (3712934) 3712934..3714391 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
MNY24_RS17910 (3714448) 3714448..3714969 - 522 Protein_3526 peptide chain release factor H -
MNY24_RS17915 (3714965) 3714965..3715171 - 207 Protein_3527 RtcB family protein -
MNY24_RS17920 (3715489) 3715489..3715941 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
MNY24_RS17925 (3715951) 3715951..3716349 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
MNY24_RS17930 (3716352) 3716352..3716645 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
MNY24_RS17935 (3716697) 3716697..3717752 - 1056 WP_001226164.1 DNA polymerase IV -
MNY24_RS17940 (3717823) 3717823..3718608 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
MNY24_RS17945 (3718580) 3718580..3720292 + 1713 Protein_3533 flagellar biosynthesis protein FlhA -
MNY24_RS17950 (3720516) 3720516..3721013 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T237869 WP_001263489.1 NZ_CP093226:c3716349-3715951 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T237869 NZ_LR882990:2769108-2769215 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT237869 WP_000554758.1 NZ_CP093226:c3716645-3716352 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT237869 NZ_LR882990:c2769055-2768994 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References