Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 611448..612247 Replicon chromosome
Accession NZ_CP093226
Organism Escherichia coli strain EC-16-35

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag MNY24_RS02980 Protein ID WP_000347275.1
Coordinates 611448..611912 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MNY24_RS02985 Protein ID WP_001307405.1
Coordinates 611912..612247 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY24_RS02950 (606449) 606449..606883 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MNY24_RS02955 (606901) 606901..607779 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
MNY24_RS02960 (607769) 607769..608548 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
MNY24_RS02965 (608559) 608559..609032 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
MNY24_RS02970 (609055) 609055..610335 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
MNY24_RS02975 (610584) 610584..611393 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MNY24_RS02980 (611448) 611448..611912 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MNY24_RS02985 (611912) 611912..612247 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MNY24_RS02990 (612396) 612396..613967 - 1572 WP_001273752.1 galactarate dehydratase -
MNY24_RS02995 (614342) 614342..615676 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MNY24_RS03000 (615692) 615692..616462 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T237850 WP_000347275.1 NZ_CP093226:c611912-611448 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T237850 NZ_LR882978:3719364-3719582 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT237850 WP_001307405.1 NZ_CP093226:c612247-611912 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT237850 NZ_LR882978:3718964-3719338 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References