Detailed information of TA system TA237750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 7686..8209
• Replicon: plasmid p1
• Accession: NZ_CP093174
• Organism: Staphylococcus epidermidis strain TMDU-137

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: C5QR05
• Locus tag: MNU44_RS11985
• Protein ID: WP_002441971.1
• Coordinates: 7943..8209 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A8I0W7P8
• Locus tag: MNU44_RS11980
• Protein ID: WP_002502803.1
• Coordinates: 7686..7943 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNU44_RS11950 (MNU44_11940)	2853..3131	-	279	WP_049325206.1	hypothetical protein	-
MNU44_RS11955 (MNU44_11945)	3706..4110	-	405	WP_002502797.1	hypothetical protein	-
MNU44_RS11960 (MNU44_11950)	4531..5193	-	663	WP_254105785.1	hypothetical protein	-
MNU44_RS11965 (MNU44_11955)	5502..5642	-	141	WP_230691398.1	hypothetical protein	-
MNU44_RS11970 (MNU44_11960)	6392..6790	-	399	WP_002502801.1	YolD-like family protein	-
MNU44_RS11975 (MNU44_11965)	6950..7507	+	558	WP_049325179.1	hypothetical protein	-
MNU44_RS11980 (MNU44_11970)	7686..7943	+	258	WP_002502803.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MNU44_RS11985 (MNU44_11975)	7943..8209	+	267	WP_002441971.1	Txe/YoeB family addiction module toxin	Toxin
MNU44_RS11990 (MNU44_11980)	8226..8396	+	171	WP_002502804.1	hypothetical protein	-
MNU44_RS11995 (MNU44_11985)	8893..9423	+	531	WP_002502805.1	hypothetical protein	-
MNU44_RS12000 (MNU44_11990)	9718..10257	+	540	WP_049325182.1	hypothetical protein	-
MNU44_RS12005 (MNU44_11995)	10250..10798	+	549	WP_049325184.1	recombinase family protein	-
MNU44_RS12010 (MNU44_12000)	10823..11593	+	771	WP_002502807.1	CPBP family intramembrane metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48107	48107

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (4-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-67)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10692.34 Da
• Isoelectric Point: 10.2282

>T237750 WP_002441971.1 NZ_CP093174:7943-8209 [Staphylococcus epidermidis]
MAKYKINIKNSAKTDLKKIKRSHLQEQFLKIVEILKYDPYQQSQSFEKLHPKHLGRYSRRINHQHRVVYTIDEENKEVLI
LAAWSHYE

Nucleotide

• Length: 267 bp

>T237750 NZ_LR882500:2811788-2812213 [Mycobacterium tuberculosis variant microti]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9955.14 Da
• Isoelectric Point: 5.1750

>AT237750 WP_002502803.1 NZ_CP093174:7686-7943 [Staphylococcus epidermidis]
MKSKTPTEARKDFYQLLKRVNSDHKPVYIEGKNDESNAVIIGMEDWRSIKETIYLEATGTMQKVREREQDNSGYTSIDDI
DWARL

Nucleotide

• Length: 258 bp

>AT237750 NZ_LR882500:2811561-2811782 [Mycobacterium tuberculosis variant microti]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA