Detailed information of TA system TA023775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 297161..297674
• Replicon: chromosome
• Accession: NC_012633
• Organism: Rickettsia africae ESF-5

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: H8LMC5
• Locus tag: RAF_RS01510
• Protein ID: WP_004996425.1
• Coordinates: 297414..297674 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: C3PMR0
• Locus tag: RAF_RS01505
• Protein ID: WP_012719480.1
• Coordinates: 297161..297403 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RAF_RS01485	292188..293957	-	1770	WP_012719477.1	ABC transporter ATP-binding protein/permease	-
RAF_RS01490	294138..294410	+	273	WP_010976947.1	DUF2671 domain-containing protein	-
RAF_RS01495	294562..295932	+	1371	WP_012719478.1	cytochrome ubiquinol oxidase subunit I	-
RAF_RS01500	295940..296959	+	1020	WP_012719479.1	cytochrome d ubiquinol oxidase subunit II	-
RAF_RS01505	297161..297403	+	243	WP_012719480.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RAF_RS01510	297414..297674	+	261	WP_004996425.1	Txe/YoeB family addiction module toxin	Toxin
RAF_RS01515	297667..298515	+	849	WP_012719481.1	glycosyltransferase family 2 protein	-
RAF_RS01520	298589..299827	+	1239	WP_041471817.1	insulinase family protein	-
RAF_RS01525	299981..300691	+	711	WP_004996420.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
RAF_RS01530	300911..302498	+	1588	Protein_314	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (3-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-63)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10352.19 Da
• Isoelectric Point: 9.9055

>T23775 WP_004996425.1 NC_012633:297414-297674 [Rickettsia africae ESF-5]
MYIIRYTIQAQKDAKKIVQAGLKNKVEVLLNIVSTDPWKIYPPYEKLVGDFSGCYSRRINIQHRLVYEVYKQEKVVKILR
MYTYYE

Nucleotide

• Length: 261 bp

>T23775 NC_012633:297414-297674 [Rickettsia africae ESF-5]
ATGTATATAATTCGTTATACTATACAAGCACAAAAAGATGCTAAGAAAATAGTGCAAGCCGGTTTAAAGAATAAAGTAGA
AGTTTTATTAAACATAGTTAGTACTGATCCTTGGAAAATTTACCCTCCATATGAAAAGCTCGTTGGTGACTTTAGCGGTT
GCTATTCAAGAAGAATTAATATTCAACATAGGTTGGTTTATGAAGTTTATAAACAAGAAAAAGTTGTAAAAATTCTTAGA
ATGTATACGTATTATGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9465.93 Da
• Isoelectric Point: 7.1617

>AT23775 WP_012719480.1 NC_012633:297161-297403 [Rickettsia africae ESF-5]
MEIYNTSEARSKLYKLIDYVSDVHKPVYIKGKRNNVVIISEEDYRNMEETLYLLSIPNMHKFIIEGRAEPIAKCSDKLNW

Nucleotide

• Length: 243 bp

>AT23775 NC_012633:297161-297403 [Rickettsia africae ESF-5]
ATGGAAATATATAATACAAGCGAAGCAAGAAGTAAGCTTTATAAACTTATAGATTATGTTTCTGATGTACACAAACCTGT
TTATATTAAAGGTAAAAGAAATAATGTAGTTATAATTTCAGAAGAAGATTATCGTAATATGGAAGAAACTTTATATTTAT
TATCTATTCCAAATATGCATAAGTTTATAATTGAAGGACGAGCAGAGCCTATAGCAAAGTGTAGCGATAAATTAAATTGG
TAA

Structures

Toxin

• AlphaFold DB: H8LMC5

Antitoxin

• AlphaFold DB: C3PMR0