Detailed information of TA system TA237724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1739839..1740499
• Replicon: chromosome
• Accession: NZ_CP093154
• Organism: Enterobacter asburiae strain 5549

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MND42_RS08495
• Protein ID: WP_100245677.1
• Coordinates: 1739839..1740192 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1Z3MX79
• Locus tag: MND42_RS08500
• Protein ID: WP_029741460.1
• Coordinates: 1740197..1740499 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MND42_RS08475 (MND42_08475)	1735981..1736898	+	918	WP_008499475.1	nitrogen assimilation transcriptional regulator NAC	-
MND42_RS08480 (MND42_08480)	1736995..1737945	+	951	WP_010432106.1	HTH-type transcriptional regulator Cbl	-
MND42_RS08485 (MND42_08485)	1737989..1738597	-	609	WP_057061635.1	glutathione S-transferase family protein	-
MND42_RS08490 (MND42_08490)	1738768..1739661	+	894	WP_047063523.1	LysR substrate-binding domain-containing protein	-
MND42_RS08495 (MND42_08495)	1739839..1740192	+	354	WP_100245677.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MND42_RS08500 (MND42_08500)	1740197..1740499	+	303	WP_029741460.1	XRE family transcriptional regulator	Antitoxin
MND42_RS08505 (MND42_08505)	1740576..1741496	-	921	WP_045403136.1	LysR family transcriptional regulator	-
MND42_RS08510 (MND42_08510)	1741638..1742027	+	390	WP_023312340.1	RidA family protein	-
MND42_RS08520 (MND42_08520)	1742351..1744540	-	2190	WP_045403139.1	TonB-dependent siderophore receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	iutA / iucC / iucA	1662027..1757414	95387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13432.23 Da
• Isoelectric Point: 9.0310

>T237724 WP_100245677.1 NZ_CP093154:1739839-1740192 [Enterobacter asburiae]
VSAINTTDRFDRWFTSLDDTERASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLNTLKERE

Nucleotide

• Length: 354 bp

>T237724 NZ_LR882500:c757046-756738 [Mycobacterium tuberculosis variant microti]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11050.83 Da
• Isoelectric Point: 5.7316

>AT237724 WP_029741460.1 NZ_CP093154:1740197-1740499 [Enterobacter asburiae]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT237724 NZ_LR882500:c757278-757033 [Mycobacterium tuberculosis variant microti]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MX79