Detailed information of TA system TA237721

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 139009..139262
• Replicon: plasmid pCY814036-KPC2
• Accession: NZ_CP093153
• Organism: Klebsiella pneumoniae strain CY814036

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: MMZ10_RS28730
• Protein ID: WP_001312851.1
• Coordinates: 139113..139262 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	139009..139068 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMZ10_RS28695 (134669)	134669..134734	-	66	Protein_174	helix-turn-helix domain-containing protein	-
MMZ10_RS28700 (134787)	134787..135491	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
MMZ10_RS28705 (135516)	135516..135716	+	201	WP_072354025.1	hypothetical protein	-
MMZ10_RS28710 (135736)	135736..136482	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
MMZ10_RS28715 (136537)	136537..137097	+	561	WP_000139328.1	fertility inhibition protein FinO	-
MMZ10_RS28720 (137229)	137229..137429	+	201	WP_015059022.1	hypothetical protein	-
MMZ10_RS28725 (137815)	137815..138414	+	600	WP_032083981.1	PIN domain-containing protein	-
- (139009)	139009..139068	-	60	NuclAT_1	-	Antitoxin
- (139009)	139009..139068	-	60	NuclAT_1	-	Antitoxin
- (139009)	139009..139068	-	60	NuclAT_1	-	Antitoxin
- (139009)	139009..139068	-	60	NuclAT_1	-	Antitoxin
MMZ10_RS28730 (139113)	139113..139262	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
MMZ10_RS28735 (139546)	139546..139794	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB	-	1..140105	140105

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T237721 WP_001312851.1 NZ_CP093153:139113-139262 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T237721 NZ_LR882500:718184-718579 [Mycobacterium tuberculosis variant microti]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT237721 NZ_CP093153:c139068-139009 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin